2C4B

Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d2c4bb1	Alpha and beta proteins (a+b)	Microbial ribonucleases	Microbial ribonucleases	Bacterial ribonucleases	Barnase	(Bacillus amyloliquefaciens ) [TaxId: 1390 ], (Momordica cochinchinensis ) [TaxId: 3674 ], (Ecballium elaterium ) [TaxId: 3679 ],	SCOPe (2.08)
B	d2c4bb2	Small proteins	Knottins (small inhibitors, toxins, lectins)	Plant inhibitors of proteinases and amylases	Plant inhibitors of proteinases and amylases	Trypsin inhibitor	(Bacillus amyloliquefaciens ) [TaxId: 1390 ], (Momordica cochinchinensis ) [TaxId: 3674 ], (Ecballium elaterium ) [TaxId: 3679 ],	SCOPe (2.08)
A	d2c4ba1	Alpha and beta proteins (a+b)	Microbial ribonucleases	Microbial ribonucleases	Bacterial ribonucleases	Barnase	(Bacillus amyloliquefaciens ) [TaxId: 1390 ], (Momordica cochinchinensis ) [TaxId: 3674 ], (Ecballium elaterium ) [TaxId: 3679 ],	SCOPe (2.08)
A	d2c4ba2	Small proteins	Knottins (small inhibitors, toxins, lectins)	Plant inhibitors of proteinases and amylases	Plant inhibitors of proteinases and amylases	Trypsin inhibitor	(Bacillus amyloliquefaciens ) [TaxId: 1390 ], (Momordica cochinchinensis ) [TaxId: 3674 ], (Ecballium elaterium ) [TaxId: 3679 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Microbial ribonucleases	8033554	3001847	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Microbial ribonucleases	8033554	3001847	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Ribonuclease	e2c4bB1	A: a+b two layers	X: Microbial ribonucleases-like	H: Microbial ribonucleases (From Topology)	T: Microbial ribonucleases	F: Ribonuclease	ECOD (1.6)
B	Squash	e2c4bB2	A: few secondary structure elements	X: omega toxin-like	H: omega toxin-related	T: omega toxin-related	F: Squash	ECOD (1.6)
A	Ribonuclease	e2c4bA1	A: a+b two layers	X: Microbial ribonucleases-like	H: Microbial ribonucleases (From Topology)	T: Microbial ribonucleases	F: Ribonuclease	ECOD (1.6)
A	Squash	e2c4bA2	A: few secondary structure elements	X: omega toxin-like	H: omega toxin-related	T: omega toxin-related	F: Squash	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.10.450.30	Alpha Beta	Roll	Nuclear Transport Factor 2	Chain: A,	CATH (4.3.0)
A	3.10.450.30	Alpha Beta	Roll	Nuclear Transport Factor 2	Chain: A,	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00545	ribonuclease (Ribonuclease)	ribonuclease	This enzyme hydrolyses RNA and oligoribonucleotides.	Domain
A, B	PF00299	Squash family serine protease inhibitor (Squash)	Squash family serine protease inhibitor	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	BARNASE MCOEETI FUSION	enzyme regulator activity molecular function inhibitor activity peptidase regulator activity serine-type endopeptidase inhibitor activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity enzyme regulator activity molecular function inhibitor activity peptidase regulator activity serine-type endopeptidase inhibitor activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding	negative regulation of hydrolase activity regulation of cellular process negative regulation of proteolysis regulation of serine-type endopeptidase activity regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity negative regulation of endopeptidase activity regulation of primary metabolic process negative regulation of peptidase activity regulation of proteolysis regulation of serine-type peptidase activity proteolysis regulation of endopeptidase activity negative regulation of cellular process negative regulation of hydrolase activity regulation of cellular process negative regulation of proteolysis regulation of serine-type endopeptidase activity regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity negative regulation of endopeptidase activity regulation of primary metabolic process negative regulation of peptidase activity regulation of proteolysis regulation of serine-type peptidase activity proteolysis regulation of endopeptidase activity negative regulation of cellular process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process negative regulation of serine-type endopeptidase activity regulation of biological process negative regulation of serine-type peptidase activity regulation of protein metabolic process negative regulation of metabolic process regulation of peptidase activity biological regulation negative regulation of molecular function primary metabolic process regulation of macromolecule metabolic process regulation of molecular function regulation of hydrolase activity metabolic process cellular process macromolecule metabolic process regulation of metabolic process response to stimulus defense response response to stress	cellular anatomical structure extracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR011052	Proteinase/amylase inhibitor domain superfamily	Homologous Superfamily
A, B	IPR000737	Proteinase inhibitor I7, squash	Family
A, B	IPR000026	Guanine-specific ribonuclease N1-like	Family
A, B	IPR053753	Ribonuclease N1/T1-like	Homologous Superfamily
A, B	IPR001887	Barnase	Family
A, B	IPR016191	Ribonuclease/ribotoxin	Homologous Superfamily
A, B	IPR011052	Proteinase/amylase inhibitor domain superfamily	Homologous Superfamily
A, B	IPR000737	Proteinase inhibitor I7, squash	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.