2KYM | pdb_00002kym

Solution structure of the Bem1p SH3-CI domain from L.elongisporus in complex with Ste20p peptide

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SH3_1	e2kymA1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: SH3_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.30.40	Mainly Beta	Roll	SH3 type barrels.	SH3 Domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00018	SH3 domain (SH3_1)	SH3 domain	SH3 (Src homology 3) domains are often indicative of a protein involved in signal transduction related to cytoskeletal organisation. First described in the Src cytoplasmic tyrosine kinase Swiss:P12931. The structure is a partly opened beta barrel.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Bud emergence protein 1	binding phospholipid binding lipid binding phosphatidylinositol-3-phosphate binding phosphatidylinositol phosphate binding phosphatidylinositol binding protein-macromolecule adaptor activity molecular adaptor activity	developmental process anatomical structure morphogenesis sexual reproduction reproductive process anatomical structure development cell morphogenesis involved in conjugation with cellular fusion cell morphogenesis conjugation with cellular fusion maintenance of protein location protein localization localization cellular localization cellular macromolecule localization macromolecule localization developmental process anatomical structure morphogenesis sexual reproduction reproductive process anatomical structure development cell morphogenesis involved in conjugation with cellular fusion cell morphogenesis conjugation with cellular fusion maintenance of protein location protein localization localization cellular localization cellular macromolecule localization macromolecule localization cellular process maintenance of location growth filamentous growth positive regulation of organelle organization regulation of biological process regulation of cellular component organization regulation of cellular process positive regulation of cellular component organization vacuole organization positive regulation of vacuole organization positive regulation of vacuole fusion, non-autophagic biological regulation positive regulation of biological process regulation of vacuole organization regulation of organelle organization cellular component organization organelle fusion organelle organization vacuole fusion vacuole fusion, non-autophagic regulation of vacuole fusion, non-autophagic cellular component organization or biogenesis positive regulation of cellular process	cellular anatomical structure incipient cellular bud site site of polarized growth cellular bud cellular bud tip cell periphery cell cortex intracellular anatomical structure cytoplasm cell projection mating projection cell tip mating projection tip cell pole cellular anatomical structure incipient cellular bud site site of polarized growth cellular bud cellular bud tip cell periphery cell cortex intracellular anatomical structure cytoplasm cell projection mating projection cell tip mating projection tip cell pole plasma membrane bounded cell projection cellular bud neck protein kinase complex transferase complex, transferring phosphorus-containing groups PAR polarity complex protein-containing complex catalytic complex serine/threonine protein kinase complex transferase complex
B	Peptide form Serine/threonine-protein kinase STE20	protein serine/threonine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups histone H2AS121 kinase activity histone modifying activity histone H2A kinase activity catalytic activity histone kinase activity AMP-activated protein kinase activity DNA-dependent protein kinase activity protein serine/threonine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups histone H2AS121 kinase activity histone modifying activity histone H2A kinase activity catalytic activity histone kinase activity AMP-activated protein kinase activity DNA-dependent protein kinase activity histone H3T45 kinase activity histone H3 kinase activity eukaryotic translation initiation factor 2alpha kinase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding RNA binding nucleic acid binding mRNA binding histone H3T6 kinase activity histone H3T3 kinase activity histone H2BS14 kinase activity histone H2B kinase activity histone H3S57 kinase activity Rho-dependent protein serine/threonine kinase activity MAP kinase kinase kinase kinase activity histone H2AT120 kinase activity histone H2AXS139 kinase activity histone H2AX kinase activity histone H4S1 kinase activity histone H4 kinase activity protein serine kinase activity histone H3T11 kinase activity histone H3S28 kinase activity 3-phosphoinositide-dependent protein kinase activity histone H2BS36 kinase activity histone H2AS1 kinase activity ribosomal protein S6 kinase activity histone H3S10 kinase activity	pseudohyphal growth growth cellular process growth of unicellular organism as a thread of attached cells filamentous growth cell growth filamentous growth of a population of unicellular organisms cytoskeleton-dependent cytokinesis reproductive process in single-celled organism reproductive process mitotic cytokinesis cytokinesis establishment or maintenance of cell polarity mitotic cell cycle pseudohyphal growth growth cellular process growth of unicellular organism as a thread of attached cells filamentous growth cell growth filamentous growth of a population of unicellular organisms cytoskeleton-dependent cytokinesis reproductive process in single-celled organism reproductive process mitotic cytokinesis cytokinesis establishment or maintenance of cell polarity mitotic cell cycle asexual reproduction establishment of cell polarity mitotic cell cycle process cell cycle cell division cellular bud site selection cell budding bipolar cellular bud site selection cell cycle process invasive growth in response to glucose limitation invasive filamentous growth regulation of cell cycle phase transition regulation of cellular process cell cycle phase transition exit from mitosis cellular component organization regulation of mitotic cell cycle cellular component organization or biogenesis regulation of cell cycle regulation of biological process mitotic nuclear division regulation of cell cycle process organelle fission biological regulation regulation of mitotic cell cycle phase transition nuclear division organelle organization regulation of exit from mitosis mitotic cell cycle phase transition positive regulation of programmed cell death regulation of apoptotic process positive regulation of biological process regulation of programmed cell death cell death positive regulation of apoptotic process apoptotic process programmed cell death positive regulation of cellular process cellular response to stimulus regulation of signaling response to stimulus signaling regulation of signal transduction regulation of MAPK cascade intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette MAPK cascade regulation of cell communication signal transduction cellular response to nutrient levels response to nutrient levels cellular response to stress response to stress response to starvation cellular response to starvation cellular component biogenesis cellular component assembly stress granule assembly organelle assembly membraneless organelle assembly sexual reproduction regulation of reproductive process regulation of conjugation with cellular fusion response to pheromone cellular response to pheromone positive regulation of conjugation with cellular fusion positive regulation of reproductive process pheromone-dependent signal transduction involved in conjugation with cellular fusion signal transduction involved in positive regulation of conjugation with cellular fusion conjugation with cellular fusion response to pheromone triggering conjugation with cellular fusion cellular response to chemical stimulus response to chemical negative regulation of biological process RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process response to osmotic stress osmosensory signaling pathway via Sho1 osmosensor cellular response to chemical stress cellular response to osmotic stress cellular response to environmental stimulus osmosensory signaling pathway response to abiotic stimulus cellular response to abiotic stimulus localization transport macromolecule localization lipid localization lipid transport sterol transport organic hydroxy compound transport sterol import establishment of localization signal transduction involved in filamentous growth budding cell bud growth budding cell apical bud growth cellular response to oxidative stress response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to hydrogen peroxide response to reactive oxygen species response to hydrogen peroxide response to oxidative stress cellular response to reactive oxygen species vacuole organization organelle inheritance vacuole inheritance negative regulation of gene expression regulation of localization negative regulation of sterol transport regulation of lipid transport negative regulation of transport negative regulation of lipid localization negative regulation of sterol import regulation of sterol transport negative regulation of lipid transport regulation of transport regulation of sterol import regulation of lipid localization	cellular anatomical structure incipient cellular bud site site of polarized growth cell projection mating projection cell tip mating projection tip cell pole plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cell periphery plasma membrane membrane cellular anatomical structure incipient cellular bud site site of polarized growth cell projection mating projection cell tip mating projection tip cell pole plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cell periphery plasma membrane membrane membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR051228	NADPH Oxidase and PX Domain-Containing	Family
A	IPR001683	Phox homology	Domain
A	IPR036871	PX domain superfamily	Homologous Superfamily
A	IPR001452	SH3 domain	Domain
A	IPR036028	SH3-like domain superfamily	Homologous Superfamily
A	IPR035550	Bem1/Scd2, PX domain	Domain
A	IPR035549	Bem1/Scd2, SH3 domain 2	Domain
B	IPR000719	Protein kinase domain	Domain
B	IPR036936	CRIB domain superfamily	Homologous Superfamily
B	IPR000095	CRIB domain	Domain
B	IPR017441	Protein kinase, ATP binding site	Binding Site
B	IPR051931	Serine/threonine-protein kinase PAK 3-like	Family
B	IPR033923	p21 activated kinase binding domain	Domain
B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
B	IPR008271	Serine/threonine-protein kinase, active site	Active Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.