Annotations: 2P01

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	RAP domain-like	8040777	3001254	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Alpha-2-MRAP_C	e2p01A1	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: alpha-2-Macroglobulin receptor associated protein (RAP) domain 1 (From Topology)	T: alpha-2-Macroglobulin receptor associated protein (RAP) domain 1	F: Alpha-2-MRAP_C	ECOD (1.6)
A	Alpha-2-MRAP_N	e2p01A2	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: alpha-2-Macroglobulin receptor associated protein (RAP) domain 1 (From Topology)	T: alpha-2-Macroglobulin receptor associated protein (RAP) domain 1	F: Alpha-2-MRAP_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.81.10	Mainly Alpha	Up-down Bundle	Receptor-associated Protein	RAP domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF06401	Alpha-2-macroglobulin RAP, C-terminal domain (Alpha-2-MRAP_C)	Alpha-2-macroglobulin RAP, C-terminal domain	The alpha-2-macroglobulin receptor-associated protein (RAP) is a intracellular glycoprotein that binds to the 2-macroglobulin receptor and other members of the low density lipoprotein receptor family. The protein inhibits binding of all currently k ...	The alpha-2-macroglobulin receptor-associated protein (RAP) is a intracellular glycoprotein that binds to the 2-macroglobulin receptor and other members of the low density lipoprotein receptor family. The protein inhibits binding of all currently known ligands of these receptors [1]. Two different studies have provided conflicted domain boundaries [2,3].	Domain
A	PF06400	Alpha-2-macroglobulin RAP, N-terminal domain (Alpha-2-MRAP_N)	Alpha-2-macroglobulin RAP, N-terminal domain	The alpha-2-macroglobulin receptor-associated protein (RAP) is a intracellular glycoprotein that binds to the 2-macroglobulin receptor and other members of the low density lipoprotein receptor family. The protein inhibits binding of all currently k ...	The alpha-2-macroglobulin receptor-associated protein (RAP) is a intracellular glycoprotein that binds to the 2-macroglobulin receptor and other members of the low density lipoprotein receptor family. The protein inhibits binding of all currently known ligands of these receptors [1]. The N-terminal domain is predominately alpha helical [1]. Two different studies have provided conflicted domain boundaries [2,3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Alpha-2-macroglobulin receptor-associated protein	protein binding binding signaling receptor binding amide binding amyloid-beta binding peptide binding molecular transducer activity molecular function activator activity signaling receptor activity molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity molecular function inhibitor activity protein binding binding signaling receptor binding amide binding amyloid-beta binding peptide binding molecular transducer activity molecular function activator activity signaling receptor activity molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity molecular function inhibitor activity signaling receptor inhibitor activity receptor antagonist activity enzyme binding lipase binding low-density lipoprotein particle receptor binding lipoprotein particle receptor binding very-low-density lipoprotein particle receptor binding heparin binding glycosaminoglycan binding carbohydrate derivative binding sulfur compound binding	regulation of protein binding regulation of molecular function negative regulation of protein binding regulation of binding negative regulation of binding biological regulation negative regulation of molecular function multicellular organismal process regulation of biological process positive regulation of biological process positive regulation of amyloid-beta clearance positive regulation of multicellular organismal process regulation of multicellular organismal process amyloid-beta clearance regulation of protein binding regulation of molecular function negative regulation of protein binding regulation of binding negative regulation of binding biological regulation negative regulation of molecular function multicellular organismal process regulation of biological process positive regulation of biological process positive regulation of amyloid-beta clearance positive regulation of multicellular organismal process regulation of multicellular organismal process amyloid-beta clearance regulation of amyloid-beta clearance regulation of localization import into cell regulation of cellular process regulation of cellular component organization receptor internalization transport negative regulation of biological process negative regulation of transport negative regulation of endocytosis regulation of receptor internalization vesicle-mediated transport cellular component organization localization negative regulation of cellular process cellular component organization or biogenesis regulation of endocytosis negative regulation of cellular component organization regulation of transport negative regulation of receptor-mediated endocytosis regulation of receptor-mediated endocytosis regulation of vesicle-mediated transport establishment of localization cellular process endocytosis receptor-mediated endocytosis negative regulation of receptor internalization cellular response to stimulus response to stimulus signaling signal transduction cell communication regulation of signaling negative regulation of signaling negative regulation of response to stimulus regulation of signal transduction negative regulation of signal transduction negative regulation of cell communication extracellular negative regulation of signal transduction regulation of response to stimulus regulation of cell communication extracellular regulation of signal transduction very-low-density lipoprotein particle clearance regulation of plasma lipoprotein particle levels plasma lipoprotein particle clearance negative regulation of very-low-density lipoprotein particle clearance negative regulation of lipoprotein particle clearance regulation of lipoprotein particle clearance regulation of very-low-density lipoprotein particle clearance negative regulation of multicellular organismal process amyloid-beta clearance by transcytosis transcytosis negative regulation of amyloid-beta clearance	cellular anatomical structure endomembrane system membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus cis-Golgi network organelle intracellular membrane-bounded organelle cytoplasm cell surface cell periphery plasma membrane membrane cellular anatomical structure endomembrane system membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus cis-Golgi network organelle intracellular membrane-bounded organelle cytoplasm cell surface cell periphery plasma membrane membrane membrane-enclosed lumen intracellular organelle lumen endoplasmic reticulum lumen rough endoplasmic reticulum rough endoplasmic reticulum lumen organelle lumen endoplasmic reticulum Golgi lumen endosome intracellular vesicle cytoplasmic vesicle vesicle extracellular region endoplasmic reticulum-Golgi intermediate compartment endosome lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR036744	RAP domain superfamily	Homologous Superfamily
A	IPR038003	Alpha-2-macroglobulin receptor-associated protein	Family
A	IPR010483	Alpha-2-macroglobulin RAP, C-terminal	Domain
A	IPR037999	Alpha-2-macroglobulin RAP, domain 3	Domain
A	IPR009066	Alpha-2-macroglobulin receptor-associated protein, domain 1	Domain
A	IPR038001	Alpha-2-macroglobulin RAP, domain 2	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P30533	myopia myopia 23, autosomal recessive atypical teratoid rhabdoid tumor heart disease medulloblastoma ovarian cancer plasma cell myeloma dermatomyositis large cell medulloblastoma Pick disease primitive neuroectodermal tumor glioblastoma malignant glioma pilocytic astrocytoma psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.