3CX8 | pdb_00003cx8

Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GTP-gamma-S

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d3cx8b_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	Rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056782	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galpha insert domain-like	8056783	3000669	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Regulator of G-protein signaling, RGS	8062617	3000835	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e3cx8A1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	MnmE_helical_2nd	e3cx8A2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)
B	RGS-like	e3cx8B1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS-like	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF09128	Regulator of G protein signalling-like domain (RGS-like)	Regulator of G protein signalling-like domain	Members of this family adopt a structure consisting of twelve helices that fold into a compact domain that contains the overall structural scaffold observed in other RGS proteins and three additional helical elements that pack closely to it. Helices ...	Members of this family adopt a structure consisting of twelve helices that fold into a compact domain that contains the overall structural scaffold observed in other RGS proteins and three additional helical elements that pack closely to it. Helices 1-9 comprise the RGS (Pfam:PF00615) fold, in which helices 4-7 form a classic antiparallel bundle adjacent to the other helices. Like other RGS structures, helices 7 and 8 span the length of the folded domain and form essentially one continuous helix with a kink in the middle. Helices 10-12 form an apparently stable C-terminal extension of the structural domain, and although other RGS proteins lack this structure, these elements are intimately associated with the rest of the structural framework by hydrophobic interactions. Members of the family bind to active G-alpha proteins, promoting GTP hydrolysis by the alpha subunit of heterotrimeric G proteins, thereby inactivating the G protein and rapidly switching off G protein-coupled receptor signalling pathways [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Guanine nucleotide-binding protein alpha-13 subunit	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein binding signaling receptor binding G protein-coupled receptor binding D5 dopamine receptor binding dopamine receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity G protein activity ribonucleoside triphosphate phosphatase activity molecular function regulator activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity cation binding metal ion binding	positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality multicellular organismal process system process circulatory system process regulation of blood pressure blood circulation developmental process cellular developmental process cell differentiation cellular process blood vessel morphogenesis tube development positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality multicellular organismal process system process circulatory system process regulation of blood pressure blood circulation developmental process cellular developmental process cell differentiation cellular process blood vessel morphogenesis tube development angiogenesis vasculature development circulatory system development anatomical structure morphogenesis multicellular organism development tube morphogenesis anatomical structure formation involved in morphogenesis anatomical structure development blood vessel development system development cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction regulation of vascular associated smooth muscle cell migration negative regulation of locomotion vascular associated smooth muscle cell migration regulation of cell motility negative regulation of vascular associated smooth muscle cell migration regulation of smooth muscle cell migration negative regulation of biological process smooth muscle cell migration muscle cell migration negative regulation of smooth muscle cell migration regulation of locomotion regulation of cell migration negative regulation of cellular process negative regulation of cell motility cell migration cell motility locomotion negative regulation of cell migration regulation of fibroblast migration fibroblast migration ameboidal-type cell migration embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development adenylate cyclase-activating G protein-coupled receptor signaling pathway regulation of developmental process regulation of cell shape cell morphogenesis regulation of cell morphogenesis regulation of anatomical structure morphogenesis regulation of body fluid levels wound healing cell activation hemostasis blood coagulation response to stress coagulation response to wounding platelet activation branching morphogenesis of an epithelial tube epithelial tube morphogenesis epithelium development morphogenesis of an epithelium tissue development branching involved in blood vessel morphogenesis tissue morphogenesis morphogenesis of a branching structure morphogenesis of a branching epithelium regulation of cellular component organization cellular component organization cellular component biogenesis cellular component assembly regulation of postsynapse assembly cell junction assembly regulation of synapse structure or activity synapse organization cellular component organization or biogenesis regulation of synapse assembly postsynapse organization postsynapse assembly regulation of postsynapse organization nervous system development regulation of cellular component biogenesis regulation of cell junction assembly regulation of synapse organization cell junction organization synapse assembly intracellular signal transduction small GTPase-mediated signal transduction Rho protein signal transduction intracellular signaling cassette regulation of vascular associated smooth muscle cell proliferation negative regulation of cell population proliferation cell population proliferation regulation of smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell proliferation smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of cell population proliferation muscle cell proliferation vascular associated smooth muscle cell proliferation	membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle intracellular membrane-bounded organelle intracellular organelle melanosome cellular anatomical structure cytoplasmic vesicle organelle cytoplasm vesicle protein-containing complex extrinsic component of plasma membrane membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle intracellular membrane-bounded organelle intracellular organelle melanosome cellular anatomical structure cytoplasmic vesicle organelle cytoplasm vesicle protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane cytosol brush border membrane cluster of actin-based cell projections plasma membrane bounded cell projection brush border cell projection plasma membrane region cell projection membrane postsynapse synapse cell junction nucleus
B	Rho guanine nucleotide exchange factor 11	GTPase regulator activity enzyme regulator activity guanyl-nucleotide exchange factor activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein binding binding signaling receptor binding G protein-coupled receptor binding enzyme activator activity GTPase activator activity molecular function activator activity	import into cell regulation of localization nitrogen compound transport regulation of cellular process carboxylic acid transmembrane transport transport positive regulation of L-glutamate import across plasma membrane positive regulation of biological process amino acid transmembrane transport L-amino acid transport L-glutamate import positive regulation of amine transport localization regulation of L-glutamate import across plasma membrane positive regulation of transmembrane transport import into cell regulation of localization nitrogen compound transport regulation of cellular process carboxylic acid transmembrane transport transport positive regulation of L-glutamate import across plasma membrane positive regulation of biological process amino acid transmembrane transport L-amino acid transport L-glutamate import positive regulation of amine transport localization regulation of L-glutamate import across plasma membrane positive regulation of transmembrane transport regulation of amino acid transport regulation of amino acid transmembrane transport positive regulation of cellular process L-glutamate import across plasma membrane organic acid transmembrane transport regulation of biological process organic anion transport carboxylic acid transport regulation of transport import across plasma membrane biological regulation establishment of localization positive regulation of organic acid transport organic acid transport transmembrane transport amino acid transport amine transport acidic amino acid transport regulation of amino acid import across plasma membrane regulation of transmembrane transport positive regulation of transport positive regulation of amino acid transport regulation of organic acid transport dicarboxylic acid transport L-alpha-amino acid transmembrane transport cellular process amino acid import across plasma membrane regulation of amine transport L-glutamate transmembrane transport positive regulation of DNA-templated transcription RNA metabolic process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process positive regulation of nucleobase-containing compound metabolic process primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process positive regulation of RNA metabolic process regulation of gene expression biosynthetic process positive regulation of RNA biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process cellular response to stimulus response to stimulus signaling G protein-coupled receptor signaling pathway signal transduction cell communication small GTPase-mediated signal transduction Rho protein signal transduction intracellular signal transduction intracellular signaling cassette	cellular anatomical structure membrane intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000469	G-protein alpha subunit, group 12/13	Family
A	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B	IPR016137	RGS domain	Domain
B	IPR041020	ARHGEF1-like, PH domain	Domain
B	IPR001849	Pleckstrin homology domain	Domain
B	IPR037889	Rho guanine nucleotide exchange factor 11, RGS domain	Domain
B	IPR036305	RGS domain superfamily	Homologous Superfamily
B	IPR015212	Regulator of G protein signalling-like domain	Domain
B	IPR044926	RGS, subdomain 2	Homologous Superfamily
B	IPR036034	PDZ superfamily	Homologous Superfamily
B	IPR000219	Dbl homology domain	Domain
B	IPR035899	Dbl homology (DH) domain superfamily	Homologous Superfamily
B	IPR011993	PH-like domain superfamily	Homologous Superfamily
B	IPR001478	PDZ domain	Domain
B	IPR037803	Rho guanine nucleotide exchange factor 11, PH domain	Domain
B	IPR001331	Guanine-nucleotide dissociation stimulator, CDC24, conserved site	Conserved Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.