3QI2

A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 GoLoco

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galpha insert domain-like	8056780	3000669	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8056779	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Galpha insert domain-like	8056780	3000669	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e3qi2A1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	Ras	e3qi2A2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	G-alpha	e3qi2B4	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
B	Ras	e3qi2B3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain
C, D	PF02188	GoLoco motif (GoLoco)	GoLoco motif	-	Motif

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Guanine nucleotide-binding protein G(i) subunit alpha-1	D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding enzyme regulator activity cyclase regulator activity molecular function regulator activity adenylate cyclase regulator activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding enzyme regulator activity cyclase regulator activity molecular function regulator activity adenylate cyclase regulator activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding magnesium ion binding metal ion binding GDP binding G protein-coupled serotonin receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity G protein activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-inhibiting serotonin receptor signaling pathway G protein-coupled serotonin receptor signaling pathway cell communication adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-inhibiting serotonin receptor signaling pathway G protein-coupled serotonin receptor signaling pathway cell communication adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction response to hormone response to chemical response to endogenous stimulus response to peptide hormone response to oxygen-containing compound response to nitrogen compound cell division leukocyte migration immune system process mononuclear cell migration lymphocyte migration cell migration T cell migration cell motility regulation of cholesterol metabolic process sterol metabolic process regulation of lipid metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of small molecule metabolic process positive regulation of lipid metabolic process small molecule metabolic process regulation of alcohol biosynthetic process positive regulation of cholesterol biosynthetic process primary metabolic process positive regulation of steroid biosynthetic process biosynthetic process sterol biosynthetic process regulation of sterol biosynthetic process metabolic process regulation of metabolic process regulation of steroid biosynthetic process regulation of cholesterol biosynthetic process organic hydroxy compound metabolic process steroid metabolic process positive regulation of biological process cholesterol metabolic process lipid biosynthetic process alcohol metabolic process positive regulation of biosynthetic process steroid biosynthetic process positive regulation of cellular process regulation of biosynthetic process secondary alcohol biosynthetic process small molecule biosynthetic process regulation of steroid metabolic process cholesterol biosynthetic process secondary alcohol metabolic process positive regulation of lipid biosynthetic process organic hydroxy compound biosynthetic process alcohol biosynthetic process positive regulation of cholesterol metabolic process positive regulation of steroid metabolic process regulation of lipid biosynthetic process positive regulation of small molecule metabolic process positive regulation of alcohol biosynthetic process positive regulation of sterol biosynthetic process lipid metabolic process regulation of cytoskeleton organization regulation of cellular component organization microtubule cytoskeleton organization regulation of mitotic spindle organization mitotic cell cycle cellular component organization cell cycle cellular component organization or biogenesis regulation of cell cycle microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process regulation of cell cycle process regulation of microtubule-based process regulation of spindle organization regulation of organelle organization mitotic spindle organization organelle organization mitotic cell cycle process microtubule-based process cytoskeleton organization regulation of microtubule cytoskeleton organization protein localization positive regulation of protein localization to cell periphery regulation of localization protein localization to cell periphery cellular localization regulation of protein localization positive regulation of protein localization to cell cortex regulation of protein localization to cell periphery localization regulation of cellular localization regulation of protein localization to cell cortex cellular macromolecule localization macromolecule localization positive regulation of protein localization protein localization to cell cortex response to ketone response to prostaglandin response to lipid response to alcohol response to prostaglandin E regulation of signaling regulation of signal transduction intracellular signal transduction cAMP-mediated signaling regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cell communication regulation of cAMP-mediated signaling cellular response to forskolin cellular response to organic cyclic compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to forskolin cellular response to lipid cellular response to ketone response to organic cyclic compound	midbody cellular anatomical structure membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle membraneless organelle midbody cellular anatomical structure membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle membraneless organelle nuclear lumen cell periphery cell cortex cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton cytosol nucleoplasm vacuolar membrane lytic vacuole vacuole lytic vacuole membrane bounding membrane of organelle lysosomal membrane organelle membrane lysosome
C, D	Regulator of G-protein signaling 14	GTPase regulator activity enzyme regulator activity GDP-dissociation inhibitor activity nucleoside-triphosphatase regulator activity molecular function regulator activity enzyme activator activity GTPase activator activity molecular function activator activity cytoskeletal protein binding microtubule binding protein binding binding tubulin binding G-protein alpha-subunit binding GTPase regulator activity enzyme regulator activity GDP-dissociation inhibitor activity nucleoside-triphosphatase regulator activity molecular function regulator activity enzyme activator activity GTPase activator activity molecular function activator activity cytoskeletal protein binding microtubule binding protein binding binding tubulin binding G-protein alpha-subunit binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity GTPase activating protein binding kinase binding enzyme binding protein kinase binding signaling receptor complex adaptor activity protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity	behavior multicellular organismal process system process learning learning or memory nervous system process cognition cellular response to stimulus regulation of cellular process response to stimulus regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of signal transduction behavior multicellular organismal process system process learning learning or memory nervous system process cognition cellular response to stimulus regulation of cellular process response to stimulus regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of signal transduction regulation of transferase activity regulation of MAP kinase activity intracellular signal transduction regulation of primary metabolic process cell communication negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process intracellular signaling cassette negative regulation of protein modification process signal transduction phosphorus metabolic process protein modification process negative regulation of protein metabolic process regulation of biological process protein phosphorylation phosphorylation regulation of protein metabolic process negative regulation of metabolic process primary metabolic process regulation of MAPK cascade regulation of molecular function regulation of kinase activity regulation of response to stimulus negative regulation of protein phosphorylation regulation of protein serine/threonine kinase activity macromolecule modification regulation of phosphorus metabolic process metabolic process macromolecule metabolic process regulation of metabolic process regulation of protein modification process regulation of protein phosphorylation signaling negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of phosphate metabolic process negative regulation of cell communication regulation of intracellular signal transduction negative regulation of protein serine/threonine kinase activity negative regulation of cellular process protein metabolic process negative regulation of macromolecule metabolic process regulation of signaling negative regulation of response to stimulus biological regulation negative regulation of molecular function negative regulation of MAP kinase activity regulation of protein kinase activity regulation of macromolecule metabolic process negative regulation of protein kinase activity negative regulation of signal transduction negative regulation of phosphorus metabolic process MAPK cascade regulation of cell communication regulation of phosphorylation cellular process negative regulation of phosphorylation negative regulation of MAPK cascade cell division cell cycle chromosome segregation cell cycle process regulation of synaptic plasticity trans-synaptic signaling modulation of chemical synaptic transmission negative regulation of synaptic plasticity cell-cell signaling chemical synaptic transmission synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of biological quality localization cellular localization nucleocytoplasmic transport transport nuclear transport establishment of localization in cell intracellular transport establishment of localization negative regulation of ERK1 and ERK2 cascade ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade zygote asymmetric cell division asymmetric cell division cell surface receptor signaling pathway platelet-derived growth factor receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway enzyme-linked receptor protein signaling pathway G protein-coupled receptor signaling pathway regulation of G protein-coupled receptor signaling pathway positive regulation of nervous system development regulation of developmental process cellular developmental process positive regulation of biological process cell development regulation of cell differentiation regulation of neurogenesis regulation of cell development regulation of nervous system development positive regulation of developmental process multicellular organism development neurogenesis positive regulation of cell differentiation cell differentiation anatomical structure development positive regulation of neurogenesis regulation of multicellular organismal process positive regulation of cellular process system development developmental process regulation of multicellular organismal development positive regulation of cell development nervous system development positive regulation of multicellular organismal process mitotic cell cycle positive regulation of molecular function positive regulation of hydrolase activity regulation of hydrolase activity positive regulation of catalytic activity regulation of GTPase activity positive regulation of GTPase activity response to light stimulus visual learning visual behavior associative learning response to radiation response to abiotic stimulus long-term memory memory negative regulation of G protein-coupled receptor signaling pathway response to oxidative stress response to stress positive regulation of cell communication long-term synaptic potentiation positive regulation of synaptic transmission positive regulation of signaling cellular component organization organelle organization microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization spindle organization	intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton somatodendritic compartment cell projection dendrite dendritic tree intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection cytoplasm synapse cell junction glutamatergic synapse neuron spine dendritic spine postsynapse polymeric cytoskeletal fiber supramolecular fiber microtubule supramolecular polymer supramolecular complex centrosome microtubule organizing center cell periphery plasma membrane membrane postsynaptic density neuron to neuron synapse postsynaptic specialization asymmetric synapse membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body nucleoplasm organelle lumen nuclear lumen PML body

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A, B	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR001408	G-protein alpha subunit, group I	Family
C, D	IPR016137	RGS domain	Domain
C, D	IPR003116	Raf-like Ras-binding	Domain
C, D	IPR036305	RGS domain superfamily	Homologous Superfamily
C, D	IPR003109	GoLoco motif	Conserved Site
C, D	IPR037881	RGS14, RGS domain	Domain
C, D	IPR044926	RGS, subdomain 2	Homologous Superfamily
C, D	IPR024066	RGS, subdomain 1/3	Homologous Superfamily
C, D	IPR046995	Regulator of G-protein signaling 10/12/14-like	Family
C, D	IPR046992	Regulator of G-protein signaling 14, Ras-binding domain 1	Domain
C, D	IPR046993	Regulator of G protein signaling 14, Ras-binding domain 2	Domain
C, D	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P63096	substance withdrawal syndrome dissociative amnesia malignant glioma nicotine dependence pancreatic ductal adenocarcinoma acute myeloid leukemia Withdrawal Symptoms breast cancer glioblastoma intellectual disability large cell medulloblastoma malignant pancreatic neoplasm oligodendroglioma Pick disease astrocytic tumor substance withdrawal syndrome dissociative amnesia malignant glioma nicotine dependence pancreatic ductal adenocarcinoma acute myeloid leukemia Withdrawal Symptoms breast cancer glioblastoma intellectual disability large cell medulloblastoma malignant pancreatic neoplasm oligodendroglioma Pick disease astrocytic tumor breast carcinoma interstitial cystitis malignant colon neoplasm medulloblastoma ovarian cancer subependymal giant cell astrocytoma amnestic disorder atypical teratoid rhabdoid tumor breast fibroadenoma ductal breast carcinoma in situ invasive ductal breast carcinoma
C, D	Pharos: O43566	ulcerative colitis astrocytic tumor Crohn disease large cell medulloblastoma nephrolithiasis pilocytic astrocytoma rheumatoid arthritis atopic eczema glioblastoma malignant glioma oligodendroglioma primitive neuroectodermal tumor tuberculosis chronic kidney disease inflammatory bowel disease ulcerative colitis astrocytic tumor Crohn disease large cell medulloblastoma nephrolithiasis pilocytic astrocytoma rheumatoid arthritis atopic eczema glioblastoma malignant glioma oligodendroglioma primitive neuroectodermal tumor tuberculosis chronic kidney disease inflammatory bowel disease medulloblastoma osteosarcoma psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.