3VI1 | pdb_00003vi1

Crystal structure of Pseudomonas aerginosa alkaline protease complexed with Substance P(1-6)

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	beta-Roll	8042954	3001737	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Metalloproteases (zincins), catalytic domain	8035468	3001975	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	beta-Roll	8042954	3001737	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Metalloproteases (zincins), catalytic domain	8035468	3001975	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Peptidase_M10_C	e3vi1A1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Serralysin-like metalloprotease-C (From Topology)	T: Serralysin-like metalloprotease-C	F: Peptidase_M10_C	ECOD (1.6)
A	Peptidase_M10	e3vi1A2	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: Peptidase_M10	ECOD (1.6)
B	Peptidase_M10_C	e3vi1B1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Serralysin-like metalloprotease-C (From Topology)	T: Serralysin-like metalloprotease-C	F: Peptidase_M10_C	ECOD (1.6)
B	Peptidase_M10	e3vi1B2	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: Peptidase_M10	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.150.10.10	Mainly Beta	2 Solenoid	Alkaline Protease, subunit P, domain 1	Serralysin-like metalloprotease, C-terminal	CATH (4.3.0)
A	3.40.390.10	Alpha Beta	3-Layer(aba) Sandwich	Collagenase (Catalytic Domain)	Collagenase (Catalytic Domain)	CATH (4.3.0)
B	2.150.10.10	Mainly Beta	2 Solenoid	Alkaline Protease, subunit P, domain 1	Serralysin-like metalloprotease, C-terminal	CATH (4.3.0)
B	3.40.390.10	Alpha Beta	3-Layer(aba) Sandwich	Collagenase (Catalytic Domain)	Collagenase (Catalytic Domain)	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF13583	Metallo-peptidase family M12B Reprolysin-like (Reprolysin_4)	Metallo-peptidase family M12B Reprolysin-like	This zinc-binding metallo-peptidase has the characteristic binding motif HExGHxxGxxH of Reprolysin-like peptidases of family M12B.	Domain
A, B	PF00353	RTX calcium-binding nonapeptide repeat (4 copies) (HemolysinCabind)	RTX calcium-binding nonapeptide repeat (4 copies)	-	Repeat
A, B	PF08548	Peptidase M10 serralysin C terminal (Peptidase_M10_C)	Peptidase M10 serralysin C terminal	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Alkaline metalloproteinase	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity metalloendopeptidase activity metallopeptidase activity endopeptidase activity transition metal ion binding cation binding ion binding binding calcium ion binding metal ion binding small molecule binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity metalloendopeptidase activity metallopeptidase activity endopeptidase activity transition metal ion binding zinc ion binding	regulation of humoral immune response regulation of immune effector process response to stimulus negative regulation of biological process response to biotic stimulus complement activation positive regulation of immune response regulation of immune system process positive regulation of immune system process complement activation, lectin pathway regulation of biological process immune response response to other organism negative regulation of immune response regulation of humoral immune response regulation of immune effector process response to stimulus negative regulation of biological process response to biotic stimulus complement activation positive regulation of immune response regulation of immune system process positive regulation of immune system process complement activation, lectin pathway regulation of biological process immune response response to other organism negative regulation of immune response regulation of innate immune response negative regulation of immune effector process negative regulation of humoral immune response response to external stimulus immune effector process regulation of response to stimulus defense response to other organism negative regulation of response to external stimulus negative regulation of defense response regulation of immune response regulation of complement activation activation of immune response regulation of defense response humoral immune response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of response to external stimulus negative regulation of response to biotic stimulus positive regulation of biological process negative regulation of complement activation positive regulation of response to stimulus response to stress regulation of response to stress innate immune response response to external biotic stimulus regulation of complement activation, lectin pathway negative regulation of response to stimulus defense response biological regulation defense response to symbiont negative regulation of immune system process negative regulation of complement activation, lectin pathway immune system process negative regulation of innate immune response regulation of localization transport regulation of transport regulation of monoatomic ion transport regulation of sodium ion transport establishment of localization metal ion transport positive regulation of sodium ion transport localization positive regulation of transport positive regulation of monoatomic ion transport monoatomic ion transport monoatomic cation transport regulation of metal ion transport sodium ion transport adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of lymphocyte mediated immunity negative regulation of complement activation, classical pathway leukocyte mediated immunity complement activation, classical pathway negative regulation of B cell mediated immunity regulation of adaptive immune response negative regulation of adaptive immune response regulation of B cell mediated immunity negative regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains regulation of leukocyte mediated immunity negative regulation of immunoglobulin mediated immune response B cell mediated immunity regulation of complement activation, classical pathway humoral immune response mediated by circulating immunoglobulin regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains regulation of immunoglobulin mediated immune response negative regulation of humoral immune response mediated by circulating immunoglobulin immunoglobulin mediated immune response adaptive immune response regulation of lymphocyte mediated immunity negative regulation of leukocyte mediated immunity proteolysis metabolic process cellular process primary metabolic process protein metabolic process macromolecule metabolic process	cellular anatomical structure extracellular region extracellular space
C, D	Substance P1-6, RPKPQQ	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR006026	Peptidase, metallopeptidase	Domain
A, B	IPR024079	Metallopeptidase, catalytic domain superfamily	Homologous Superfamily
A, B	IPR001343	RTX calcium-binding nonapeptide repeat	Repeat
A, B	IPR016294	Peptidase M10B	Family
A, B	IPR034033	Serralysin-like metallopeptidase domain	Domain
A, B	IPR018511	Hemolysin-type calcium-binding conserved site	Conserved Site
A, B	IPR013858	Peptidase M10 serralysin, C-terminal	Domain
A, B	IPR011049	Serralysin-like metalloprotease, C-terminal	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.