4NC3

Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	G protein-coupled receptor-like	8091214	3000473	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	G protein-coupled receptor-like	8091214	3000473	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	7tm_1_2	e4nc3A4	A: alpha bundles	X: Family A G protein-coupled receptor-like (From Topology)	H: Family A G protein-coupled receptor-like (From Topology)	T: Family A G protein-coupled receptor-like	F: 7tm_1_2	ECOD (1.6)
A	Cytochrom_B562_1	e4nc3A3	A: alpha bundles	X: Four-helical up-and-down bundle	H: Cytochromes (From Topology)	T: Cytochromes	F: Cytochrom_B562_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.1070.10	Mainly Alpha	Up-down Bundle	Rhopdopsin 7-helix transmembrane proteins	Rhodopsin 7-helix transmembrane proteins	CATH (4.3.0)
A	1.20.120.10	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Cytochrome c/b562	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF07361	Cytochrome b562 (Cytochrom_B562)	Cytochrome b562	-	Family
A	PF00001	7 transmembrane receptor (rhodopsin family) (7tm_1)	7 transmembrane receptor (rhodopsin family)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Chimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562	cation binding binding ion binding serotonin binding organic cyclic compound binding amine binding heterocyclic compound binding small molecule binding neurotransmitter receptor activity molecular transducer activity signaling receptor activity serotonin receptor activity transmembrane signaling receptor activity G protein-coupled amine receptor activity cation binding binding ion binding serotonin binding organic cyclic compound binding amine binding heterocyclic compound binding small molecule binding neurotransmitter receptor activity molecular transducer activity signaling receptor activity serotonin receptor activity transmembrane signaling receptor activity G protein-coupled amine receptor activity G protein-coupled serotonin receptor activity G protein-coupled receptor activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity Gq/11-coupled serotonin receptor activity protein binding G-protein alpha-subunit binding tetrapyrrole binding heme binding electron transfer activity transition metal ion binding iron ion binding metal ion binding	response to stimulus response to chemical response to xenobiotic stimulus cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction positive regulation of canonical NF-kappaB signal transduction signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus response to stimulus response to chemical response to xenobiotic stimulus cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction positive regulation of canonical NF-kappaB signal transduction signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette signal transduction positive regulation of cellular process regulation of biological process regulation of signaling biological regulation positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of response to stimulus regulation of cell communication regulation of canonical NF-kappaB signal transduction cellular process positive regulation of signaling intestine smooth muscle contraction multicellular organismal process gastro-intestinal system smooth muscle contraction system process smooth muscle contraction phasic smooth muscle contraction muscle contraction muscle system process developmental process neural crest cell differentiation cellular developmental process animal organ development mesenchyme development tissue development stem cell differentiation cell differentiation anatomical structure development mesenchymal cell differentiation phospholipase C-activating serotonin receptor signaling pathway G protein-coupled serotonin receptor signaling pathway phospholipase C-activating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway positive regulation of ERK1 and ERK2 cascade positive regulation of MAPK cascade regulation of MAPK cascade MAPK cascade ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death regulation of lipid metabolic process glycerophospholipid metabolic process lipid biosynthetic process positive regulation of phosphate metabolic process regulation of primary metabolic process phospholipid metabolic process positive regulation of metabolic process positive regulation of biosynthetic process regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of lipid metabolic process positive regulation of phosphatidylinositol biosynthetic process phosphorus metabolic process regulation of phosphatidylinositol biosynthetic process regulation of biosynthetic process phosphatidylinositol metabolic process positive regulation of phosphorus metabolic process glycerophospholipid biosynthetic process positive regulation of phospholipid biosynthetic process regulation of phospholipid metabolic process primary metabolic process phospholipid biosynthetic process regulation of phospholipid biosynthetic process phosphatidylinositol biosynthetic process positive regulation of phospholipid metabolic process biosynthetic process positive regulation of lipid biosynthetic process glycerolipid biosynthetic process organophosphate metabolic process organophosphate biosynthetic process metabolic process regulation of phosphorus metabolic process glycerolipid metabolic process regulation of lipid biosynthetic process regulation of metabolic process lipid metabolic process cGMP-mediated signaling regulation of tube diameter regulation of tube size circulatory system process vascular process in circulatory system blood vessel diameter maintenance regulation of biological quality vasoconstriction blood circulation regulation of anatomical structure size inorganic ion homeostasis intracellular calcium ion homeostasis homeostatic process calcium ion homeostasis cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis calcium-mediated signaling stem cell development neural crest cell development cell development mesenchymal cell migration ameboidal-type cell migration cell migration cell motility neural crest cell migration positive regulation of cell division cell division regulation of cell division regulation of behavior behavior regulation of multicellular organismal process multicellular organism development anatomical structure morphogenesis embryonic morphogenesis embryo development heart development heart morphogenesis circulatory system development animal organ morphogenesis system development cellular response to nitrogen compound response to dopamine cellular response to dopamine response to monoamine response to catecholamine cellular response to catecholamine stimulus response to organic cyclic compound serotonin receptor signaling pathway cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation regulation of localization cellular localization transport monoatomic ion transmembrane transport negative regulation of sequestering of calcium ion calcium ion transport metal ion transport maintenance of location in cell monoatomic cation transmembrane transport localization monoatomic ion transport monoatomic cation transport regulation of sequestering of calcium ion calcium ion transmembrane import into cytosol sequestering of calcium ion inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport maintenance of location transmembrane transport release of sequestered calcium ion into cytosol calcium ion transmembrane transport muscle hypertrophy cardiac muscle hypertrophy striated muscle hypertrophy trans-synaptic signaling synaptic signaling anterograde trans-synaptic signaling cell-cell signaling chemical synaptic transmission positive regulation of macromolecule biosynthetic process cytokine production regulation of cytokine production positive regulation of macromolecule metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression positive regulation of gene expression positive regulation of multicellular organismal process regulation of macromolecule biosynthetic process macromolecule metabolic process positive regulation of cytokine production endothelial cell proliferation positive regulation of epithelial cell proliferation regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation regulation of epithelial cell proliferation epithelial cell proliferation G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger electron transport chain generation of precursor metabolites and energy	protein-containing complex G protein-coupled serotonin receptor complex receptor complex cell periphery cellular anatomical structure plasma membrane membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection intracellular anatomical structure protein-containing complex G protein-coupled serotonin receptor complex receptor complex cell periphery cellular anatomical structure plasma membrane membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection intracellular anatomical structure cytoplasm membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen synapse cell junction periplasmic space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000276	G protein-coupled receptor, rhodopsin-like	Family
A	IPR017452	GPCR, rhodopsin-like, 7TM	Domain
A	IPR000482	5-Hydroxytryptamine 2B receptor	Family
A	IPR002231	5-hydroxytryptamine receptor family	Family
A	IPR010980	Cytochrome c/b562	Homologous Superfamily
A	IPR009155	Cytochrome b562	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P41595	congestive heart failure heart valve disease pulmonary hypertension Cardiomegaly hypertensive disorder psoriasis acromegaly amenorrhea invasive ductal breast carcinoma malignant mesothelioma pulmonary arterial hypertension adrenal cortex carcinoma migraine disorder osteosarcoma heart failure congestive heart failure heart valve disease pulmonary hypertension Cardiomegaly hypertensive disorder psoriasis acromegaly amenorrhea invasive ductal breast carcinoma malignant mesothelioma pulmonary arterial hypertension adrenal cortex carcinoma migraine disorder osteosarcoma heart failure major depressive disorder

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	G Protein-Coupled Receptors: Class A	5-HT_2B serotonin receptor

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.