5A6J

GH20C, Beta-hexosaminidase from Streptococcus pneumoniae

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	GcnA_C	e5a6jB3	A: alpha bundles	X: Hyaluronidase domain-like	H: Hyaluronidase post-catalytic domain-like (From Topology)	T: Hyaluronidase post-catalytic domain-like	F: GcnA_C	ECOD (1.6)
B	Glyco_hydro_20	e5a6jB2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_20	ECOD (1.6)
B	GcnA_N	e5a6jB1	A: mixed a+b and a/b	X: Zincin-like	H: beta-N-acetylhexosaminidase-like domain (From Topology)	T: beta-N-acetylhexosaminidase-like domain	F: GcnA_N	ECOD (1.6)
C	GcnA_C	e5a6jC1	A: alpha bundles	X: Hyaluronidase domain-like	H: Hyaluronidase post-catalytic domain-like (From Topology)	T: Hyaluronidase post-catalytic domain-like	F: GcnA_C	ECOD (1.6)
C	Glyco_hydro_20	e5a6jC2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_20	ECOD (1.6)
C	GcnA_N	e5a6jC3	A: mixed a+b and a/b	X: Zincin-like	H: beta-N-acetylhexosaminidase-like domain (From Topology)	T: beta-N-acetylhexosaminidase-like domain	F: GcnA_N	ECOD (1.6)
A	GcnA_C	e5a6jA1	A: alpha bundles	X: Hyaluronidase domain-like	H: Hyaluronidase post-catalytic domain-like (From Topology)	T: Hyaluronidase post-catalytic domain-like	F: GcnA_C	ECOD (1.6)
A	Glyco_hydro_20	e5a6jA2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_20	ECOD (1.6)
A	GcnA_N	e5a6jA3	A: mixed a+b and a/b	X: Zincin-like	H: beta-N-acetylhexosaminidase-like domain (From Topology)	T: beta-N-acetylhexosaminidase-like domain	F: GcnA_N	ECOD (1.6)
D	GcnA_C	e5a6jD3	A: alpha bundles	X: Hyaluronidase domain-like	H: Hyaluronidase post-catalytic domain-like (From Topology)	T: Hyaluronidase post-catalytic domain-like	F: GcnA_C	ECOD (1.6)
D	Glyco_hydro_20	e5a6jD2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_20	ECOD (1.6)
D	GcnA_N	e5a6jD1	A: mixed a+b and a/b	X: Zincin-like	H: beta-N-acetylhexosaminidase-like domain (From Topology)	T: beta-N-acetylhexosaminidase-like domain	F: GcnA_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.30.160.230	Alpha Beta	2-Layer Sandwich	Double Stranded RNA Binding Domain	N-acetyl-beta-d-glucosaminidase	CATH (4.3.0)
B	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
B	1.20.120.670	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	N-acetyl-b-d-glucoasminidase	CATH (4.3.0)
C	3.30.160.230	Alpha Beta	2-Layer Sandwich	Double Stranded RNA Binding Domain	N-acetyl-beta-d-glucosaminidase	CATH (4.3.0)
C	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
C	1.20.120.670	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	N-acetyl-b-d-glucoasminidase	CATH (4.3.0)
A	3.30.160.230	Alpha Beta	2-Layer Sandwich	Double Stranded RNA Binding Domain	N-acetyl-beta-d-glucosaminidase	CATH (4.3.0)
A	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
A	1.20.120.670	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	N-acetyl-b-d-glucoasminidase	CATH (4.3.0)
D	3.30.160.230	Alpha Beta	2-Layer Sandwich	Double Stranded RNA Binding Domain	N-acetyl-beta-d-glucosaminidase	CATH (4.3.0)
D	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
D	1.20.120.670	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	N-acetyl-b-d-glucoasminidase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF18088	Glycoside Hydrolase 20C C-terminal domain (Glyco_H_20C_C)	Glycoside Hydrolase 20C C-terminal domain	This is the C-terminal domain of Glycoside hydrolase 20 C (GH20C) present in S. pneumoniae. GH20C possesses the ability to hydrolyze the beta-linkages joining either N-acetylglucosamine or N-acetylgalactosamine to a wide variety of aglycon residues. ...	This is the C-terminal domain of Glycoside hydrolase 20 C (GH20C) present in S. pneumoniae. GH20C possesses the ability to hydrolyze the beta-linkages joining either N-acetylglucosamine or N-acetylgalactosamine to a wide variety of aglycon residues. The C-terminal domain is commonly known as Domain III is important in dimerization as it forms the primary interface of the dimer. However, there is presently no evidence supporting dimerization as being necessary for catalysis. Domain III is unusual among structurally characterized GH20 enzymes but in GH20 enzymes possessing domain III, dimerization seems to be a conserved feature [1].	Domain
A, B, C, D	PF00728	Glycosyl hydrolase family 20, catalytic domain (Glyco_hydro_20)	Glycosyl hydrolase family 20, catalytic domain	This domain has a TIM barrel fold.	Domain
A, B, C, D	PF18229	N-acetyl-beta-D-glucosaminidase N-terminal domain (GcnA_N)	N-acetyl-beta-D-glucosaminidase N-terminal domain	This is the N-terminal domain found in N-acetyl-beta-D-glucosaminidase (GcnA) present in Streptococcus gordonii. GcnA is a family 20 glycosidase that cleaves N-acetyl-beta-D-glucosamine and N-acetyl-beta-D-galactosamine from 4-methylumbelliferylated ...	This is the N-terminal domain found in N-acetyl-beta-D-glucosaminidase (GcnA) present in Streptococcus gordonii. GcnA is a family 20 glycosidase that cleaves N-acetyl-beta-D-glucosamine and N-acetyl-beta-D-galactosamine from 4-methylumbelliferylated substrates. Similar N-terminal domains have been observed in all family 20 glycosidases although the number of beta-sheet strands may vary from five [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	N-ACETYL-BETA-D-GLUCOSAMINIDASE	hydrolase activity, acting on glycosyl bonds hexosaminidase activity beta-N-acetylhexosaminidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity	carbohydrate metabolic process primary metabolic process metabolic process cellular process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR038901	Hexosaminidase D-like	Family
A, B, C, D	IPR041063	Glycoside Hydrolase 20C, C-terminal	Domain
A, B, C, D	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A, B, C, D	IPR041272	N-acetyl-beta-D-glucosaminidase, N-terminal	Domain
A, B, C, D	IPR015883	Glycoside hydrolase family 20, catalytic domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.