Annotations: 6NPL

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.1740.10	Mainly Alpha	Up-down Bundle	Amino acid/polyamine transporter I	Amino acid/polyamine transporter I	CATH (utative)
B	1.20.1740.10	Mainly Alpha	Up-down Bundle	Amino acid/polyamine transporter I	Amino acid/polyamine transporter I	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03522	Solute carrier family 12 (SLC12)	Solute carrier family 12	-	Family
A, B	PF00324	Amino acid permease (AA_permease)	Amino acid permease		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Solute carrier family 12 (sodium/potassium/chloride transporter), member 2	monoatomic cation transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity solute:monoatomic cation symporter activity monoatomic anion:monoatomic cation symporter activity secondary active transmembrane transporter activity chloride:monoatomic cation symporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity symporter activity inorganic anion transmembrane transporter activity monoatomic cation transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity solute:monoatomic cation symporter activity monoatomic anion:monoatomic cation symporter activity secondary active transmembrane transporter activity chloride:monoatomic cation symporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity symporter activity inorganic anion transmembrane transporter activity ammonium channel activity passive transmembrane transporter activity inorganic cation transmembrane transporter activity channel activity solute:sodium symporter activity potassium:sodium symporter activity potassium:chloride symporter activity potassium ion transmembrane transporter activity monoatomic anion:sodium symporter activity sodium ion transmembrane transporter activity sodium:chloride symporter activity sodium:potassium:chloride symporter activity metal ion transmembrane transporter activity cation binding ion binding binding metal ion binding small molecule binding identical protein binding protein binding	transmembrane transport localization nitrogen compound transport transport ammonium transmembrane transport cellular process inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport developmental process ear development sensory organ development animal organ development anatomical structure development transmembrane transport localization nitrogen compound transport transport ammonium transmembrane transport cellular process inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport developmental process ear development sensory organ development animal organ development anatomical structure development potassium ion homeostasis inorganic ion homeostasis chemical homeostasis homeostatic process monoatomic ion homeostasis monoatomic cation homeostasis monoatomic ion transmembrane transport inorganic anion transmembrane transport chloride transport chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport monoatomic ion transport inorganic anion transport ear morphogenesis embryonic organ morphogenesis multicellular organismal process embryonic morphogenesis embryo development multicellular organism development anatomical structure morphogenesis sensory organ morphogenesis inner ear development inner ear morphogenesis animal organ morphogenesis embryonic organ development swim bladder inflation swim bladder maturation anatomical structure maturation developmental maturation animal organ maturation swim bladder development regulation of cellular component size cell volume homeostasis biological regulation cellular component organization cellular homeostasis cellular component organization or biogenesis regulation of biological quality regulation of cell size regulation of anatomical structure size chloride ion homeostasis monoatomic anion homeostasis import into cell import across plasma membrane potassium ion transmembrane transport inorganic cation import across plasma membrane metal ion transport monoatomic cation transmembrane transport inorganic ion import across plasma membrane potassium ion transport potassium ion import across plasma membrane monoatomic cation transport sodium ion homeostasis sodium ion transmembrane transport sodium ion transport	cell periphery cellular anatomical structure apical part of cell membrane plasma membrane plasma membrane region apical plasma membrane basal part of cell basal plasma membrane basolateral plasma membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004841	Amino acid permease/ SLC12A domain	Domain
A, B	IPR018491	SLC12A transporter, C-terminal	Domain
A, B	IPR013612	Amino acid permease, N-terminal	Domain
A, B	IPR002444	Solute carrier family 12 member 2	Family
A, B	IPR002443	Solute carrier family 12 member 1/2	Family
A, B	IPR004842	SLC12A transporter family	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B	OPM	Transmembrane	Alpha-helical polytopic	APC (Amino acid-Polyamine-organoCation) superfamily	Cation-chloride cotransporter

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Amino Acid/Polyamine/Organocation (APC) Superfamily	NKCC1 cation-chloride cotransporter (CCC) transmembrane domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.