Annotations: 6PR3

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6pr3a1	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	automated matches	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
A	d6pr3a3	Alpha and beta proteins (a/b)	Tetrapyrrole methylase	Tetrapyrrole methylase	Tetrapyrrole methylase	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
A	d6pr3a2	Multi-domain proteins (alpha and beta)	Siroheme synthase middle domains-like	Siroheme synthase middle domains-like	automated matches	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
B	d6pr3b1	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	automated matches	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
B	d6pr3b3	Alpha and beta proteins (a/b)	Tetrapyrrole methylase	Tetrapyrrole methylase	Tetrapyrrole methylase	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)
B	d6pr3b2	Multi-domain proteins (alpha and beta)	Siroheme synthase middle domains-like	Siroheme synthase middle domains-like	automated matches	automated matches	(Salmonella enterica subsp. enterica serovar Typhimurium ) [TaxId: 90371 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Tetrapyrrole methylase	8001041	3000017	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	CysG/Met8-like	8057834	3000045	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	CysG/Met8-like	8057834	3000045	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Tetrapyrrole methylase	8001041	3000017	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CysG_dimeriser	e6pr3A5	A: alpha superhelices	X: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	H: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	T: alpha-helical domain in siroheme synthase middle domains-like	F: CysG_dimeriser	ECOD (1.6)
A	TP_methylase_C	e6pr3A1	A: a+b two layers	X: Tetrapyrrole methylase C-terminal domain-like	H: Tetrapyrrole methylase C-terminal domain (From Topology)	T: Tetrapyrrole methylase C-terminal domain	F: TP_methylase_C	ECOD (1.6)
A	Sirohm_synth_M	e6pr3A3	A: a+b duplicates or obligate multimers	X: SSH domain in siroheme synthase middle domains-like (From Topology)	H: SSH domain in siroheme synthase middle domains-like (From Topology)	T: SSH domain in siroheme synthase middle domains-like	F: Sirohm_synth_M	ECOD (1.6)
A	NAD_binding_7	e6pr3A4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: NAD_binding_7	ECOD (1.6)
A	TP_methylase_N	e6pr3A2	A: a/b three-layered sandwiches	X: Tetrapyrrole methylase N-terminal domain (From Topology)	H: Tetrapyrrole methylase N-terminal domain (From Topology)	T: Tetrapyrrole methylase N-terminal domain	F: TP_methylase_N	ECOD (1.6)
B	CysG_dimeriser	e6pr3B1	A: alpha superhelices	X: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	H: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	T: alpha-helical domain in siroheme synthase middle domains-like	F: CysG_dimeriser	ECOD (1.6)
B	TP_methylase_C	e6pr3B2	A: a+b two layers	X: Tetrapyrrole methylase C-terminal domain-like	H: Tetrapyrrole methylase C-terminal domain (From Topology)	T: Tetrapyrrole methylase C-terminal domain	F: TP_methylase_C	ECOD (1.6)
B	Sirohm_synth_M	e6pr3B3	A: a+b duplicates or obligate multimers	X: SSH domain in siroheme synthase middle domains-like (From Topology)	H: SSH domain in siroheme synthase middle domains-like (From Topology)	T: SSH domain in siroheme synthase middle domains-like	F: Sirohm_synth_M	ECOD (1.6)
B	NAD_binding_7	e6pr3B5	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: NAD_binding_7	ECOD (1.6)
B	TP_methylase_N	e6pr3B4	A: a/b three-layered sandwiches	X: Tetrapyrrole methylase N-terminal domain (From Topology)	H: Tetrapyrrole methylase N-terminal domain (From Topology)	T: Tetrapyrrole methylase N-terminal domain	F: TP_methylase_N	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF14824	Sirohaem biosynthesis protein central (Sirohm_synth_M)	Sirohaem biosynthesis protein central	This is the central domain of a multifunctional enzyme which catalyses the biosynthesis of sirohaem. Both of the catalytic activities of this enzyme (precorrin-2 dehydrogenase EC:1.3.1.76) and sirohydrochlorin ferrochelatase (EC:4.99.1.4) are located ...	This is the central domain of a multifunctional enzyme which catalyses the biosynthesis of sirohaem. Both of the catalytic activities of this enzyme (precorrin-2 dehydrogenase EC:1.3.1.76) and sirohydrochlorin ferrochelatase (EC:4.99.1.4) are located in the N-terminal domain of this enzyme, Pfam:PF13241 [1].	Domain
A, B	PF00590	Tetrapyrrole (Corrin/Porphyrin) Methylases (TP_methylase)	Tetrapyrrole (Corrin/Porphyrin) Methylases	This family uses S-AdoMet in the methylation of diverse substrates. This family includes a related group of bacterial proteins of unknown function, including Swiss:P45528. This family includes the methylase Dipthine synthase.	Domain
A, B	PF13241	Putative NAD(P)-binding (NAD_binding_7)	Putative NAD(P)-binding	This domain is found in fungi, plants, archaea and bacteria.	Domain
A, B	PF10414	Sirohaem synthase dimerisation region (CysG_dimeriser)	Sirohaem synthase dimerisation region	Bacterial sulfur metabolism depends on the iron-containing porphinoid sirohaem. CysG, S-adenosyl-L-methionine (SAM)-dependent bis-methyltransferase, dehydrogenase and ferrochelatase, synthesises sirohaem from uroporphyrinogen III via reactions which ...	Bacterial sulfur metabolism depends on the iron-containing porphinoid sirohaem. CysG, S-adenosyl-L-methionine (SAM)-dependent bis-methyltransferase, dehydrogenase and ferrochelatase, synthesises sirohaem from uroporphyrinogen III via reactions which encompass two branchpoint intermediates in tetrapyrrole biosynthesis, diverting flux first from protoporphyrin IX biosynthesis and then from cobalamin (vitamin B12) biosynthesis. CysG is a dimer of two structurally similar protomers held together asymmetrically through a number of salt-bridges across complementary residues in the CysG_dimeriser region to produce a series of active sites, accounting for CysG's multifunctionality, catalysing four diverse reactions: two SAM-dependent methylations, NAD+-dependent tetrapyrrole dehydrogenation and metal chelation. The CysG_dimeriser region holding the two protomers together is of 74 residues [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Siroheme synthase	sirohydrochlorin ferrochelatase activity lyase activity ferrochelatase activity catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor precorrin-2 dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors NAD binding binding nucleoside phosphate binding purine nucleotide binding adenyl nucleotide binding nucleotide binding sirohydrochlorin ferrochelatase activity lyase activity ferrochelatase activity catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor precorrin-2 dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors NAD binding binding nucleoside phosphate binding purine nucleotide binding adenyl nucleotide binding nucleotide binding heterocyclic compound binding small molecule binding C-methyltransferase activity transferase activity, transferring one-carbon groups transferase activity methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity uroporphyrin-III C-methyltransferase activity	heme metabolic process pigment metabolic process porphyrin-containing compound biosynthetic process tetrapyrrole metabolic process heme biosynthetic process siroheme biosynthetic process pigment biosynthetic process tetrapyrrole biosynthetic process biosynthetic process siroheme metabolic process metabolic process cellular process porphyrin-containing compound metabolic process cobalamin metabolic process heme metabolic process pigment metabolic process porphyrin-containing compound biosynthetic process tetrapyrrole metabolic process heme biosynthetic process siroheme biosynthetic process pigment biosynthetic process tetrapyrrole biosynthetic process biosynthetic process siroheme metabolic process metabolic process cellular process porphyrin-containing compound metabolic process cobalamin metabolic process cobalamin biosynthetic process water-soluble vitamin biosynthetic process small molecule biosynthetic process vitamin biosynthetic process vitamin metabolic process small molecule metabolic process water-soluble vitamin metabolic process methylation	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR035996	Tetrapyrrole methylase superfamily	Homologous Superfamily
A, B	IPR014776	Tetrapyrrole methylase, subdomain 2	Homologous Superfamily
A, B	IPR037115	Sirohaem synthase, dimerisation domain superfamily	Homologous Superfamily
A, B	IPR006366	Uroporphyrin-III C-methyltransferase	Domain
A, B	IPR014777	Tetrapyrrole methylase, subdomain 1	Homologous Superfamily
A, B	IPR012409	Sirohaem synthase	Family
A, B	IPR028281	Siroheme synthase, central domain	Domain
A, B	IPR019478	Sirohaem synthase, dimerisation domain	Domain
A, B	IPR003043	Uroporphiryn-III C-methyltransferase, conserved site	Conserved Site
A, B	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily
A, B	IPR000878	Tetrapyrrole methylase	Domain
A, B	IPR006367	Sirohaem synthase, N-terminal	Domain
A, B	IPR050161	Siroheme/cobalamin biosynthesis enzyme	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.