6U28 | pdb_00006u28

Crystal structure of 1918 NS1-ED W187A in complex with the p85-beta-iSH2 domain of human PI3K

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6u28a_	Alpha and beta proteins (a+b)	Ns1 effector domain-like	Ns1 effector domain-like	Ns1 effector domain-like	automated matches	(Influenza A virus (A/Brevig Mission/1/1918(H1N1)) ) [TaxId: 88776 ],	SCOPe (2.08)
C [auth B]	d6u28b_	Alpha and beta proteins (a+b)	Ns1 effector domain-like	Ns1 effector domain-like	Ns1 effector domain-like	automated matches	(Influenza A virus (A/Brevig Mission/1/1918(H1N1)) ) [TaxId: 88776 ],	SCOPe (2.08)
D	d6u28d1	Coiled coil proteins	Antiparallel coiled-coil	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 (iSH2) domain-like	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain-like	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d6u28d2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ns1 effector domain-like	8086492	3000759	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Ns1 effector domain-like	8086492	3000759	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	PI3K inter-SH2 coiled-coil domain	8052379	3002111	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Flu_NS1_C	e6u28A1	A: mixed a+b and a/b	X: Ns1 effector domain-like (From Topology)	H: Ns1 effector domain-like (From Topology)	T: Ns1 effector domain-like	F: Flu_NS1_C	ECOD (1.6)
C [auth B]	Flu_NS1_C	e6u28B1	A: mixed a+b and a/b	X: Ns1 effector domain-like (From Topology)	H: Ns1 effector domain-like (From Topology)	T: Ns1 effector domain-like	F: Flu_NS1_C	ECOD (1.6)
B [auth C]	PI3K_P85_iSH2	e6u28C1	A: alpha bundles	X: YscO-like	H: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain (From Topology)	T: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain	F: PI3K_P85_iSH2	ECOD (1.6)
D	PI3K_P85_iSH2	e6u28D1	A: alpha bundles	X: YscO-like	H: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain (From Topology)	T: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain	F: PI3K_P85_iSH2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.330	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (utative)
C [auth B]	3.30.420.330	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF00600	Influenza non-structural protein (NS1) (Flu_NS1)	Influenza non-structural protein (NS1)	-	Family
B [auth C], D	PF16454	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PI3K_P85_iSH2)	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain	This domain is found between the two SH2 domains in phosphatidylinositol 3-kinase regulatory subunit P85. It forms a complex with the adaptor-binding domain of phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	Non-structural protein 1	protein serine/threonine kinase activity enzyme regulator activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity enzyme inhibitor activity protein kinase regulator activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity catalytic activity protein serine/threonine kinase activity enzyme regulator activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity enzyme inhibitor activity protein kinase regulator activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity catalytic activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity RNA binding binding nucleic acid binding	symbiont-mediated perturbation of host cellular process symbiont-mediated suppression of host mRNA processing biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process symbiont-mediated perturbation of host gene expression modulation of process of another organism biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host gene expression symbiont-mediated perturbation of host defense response symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway symbiont-mediated perturbation of host immune response symbiont-mediated suppression of host signal transduction pathway symbiont-mediated perturbation of host cellular process symbiont-mediated suppression of host mRNA processing biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process symbiont-mediated perturbation of host gene expression modulation of process of another organism biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host gene expression symbiont-mediated perturbation of host defense response symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway symbiont-mediated perturbation of host immune response symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response symbiont-mediated perturbation of host innate immune response symbiont-mediated perturbation of host signal transduction pathway symbiont-mediated suppression of cytoplasmic pattern recognition receptor signaling pathway response to stimulus response to other organism symbiont-mediated perturbation of host defenses symbiont-mediated suppression of host PKR/eIFalpha signaling symbiont-mediated response to host defenses response to external stimulus response to biotic stimulus symbiont-mediated suppression of host-directed shutoff of host translation symbiont-mediated suppression of host defenses response to host response to external biotic stimulus symbiont-mediated suppression of host interferon-mediated signaling pathway symbiont-mediated suppression of host type I interferon-mediated signaling pathway	host intracellular membrane-bounded organelle host cellular component host intracellular part cellular anatomical structure host cell part host intracellular region host cell nucleus host intracellular organelle host cell cytoplasm
B [auth C], D	Phosphatidylinositol 3-kinase regulatory subunit beta	enzyme regulator activity phosphatidylinositol kinase activity kinase activity transferase activity 1-phosphatidylinositol-3-kinase regulator activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups lipid kinase activity molecular function regulator activity catalytic activity kinase regulator activity 1-phosphatidylinositol-3-kinase activity protein heterodimerization activity protein binding enzyme regulator activity phosphatidylinositol kinase activity kinase activity transferase activity 1-phosphatidylinositol-3-kinase regulator activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups lipid kinase activity molecular function regulator activity catalytic activity kinase regulator activity 1-phosphatidylinositol-3-kinase activity protein heterodimerization activity protein binding binding protein dimerization activity phosphatidylinositol 3-kinase binding phosphatidylinositol 3-kinase regulatory subunit binding phosphoprotein binding phosphotyrosine residue binding protein phosphorylated amino acid binding protein phosphatase binding phosphatase binding enzyme binding protein tyrosine kinase binding kinase binding signaling receptor binding receptor tyrosine kinase binding protein kinase binding	cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis developmental process multicellular organismal process cellular developmental process hemopoiesis B cell activation cell activation cell development cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis developmental process multicellular organismal process cellular developmental process hemopoiesis B cell activation cell activation cell development mononuclear cell differentiation lymphocyte differentiation lymphocyte activation leukocyte activation B cell differentiation cell differentiation immune system process anatomical structure development cellular process leukocyte differentiation regulation of cytoskeleton organization regulation of cellular process regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly cellular component biogenesis cellular component organization cellular component assembly actin filament bundle organization actin filament-based process cellular component organization or biogenesis stress fiber assembly regulation of biological process regulation of actin filament organization actin cytoskeleton organization actomyosin structure organization biological regulation actin filament organization regulation of cellular component biogenesis regulation of organelle organization regulation of stress fiber assembly organelle organization contractile actin filament bundle assembly regulation of actomyosin structure organization regulation of actin cytoskeleton organization actin filament bundle assembly regulation of supramolecular fiber organization supramolecular fiber organization cytoskeleton organization response to stimulus immune response cellular response to stimulus signaling intracellular signal transduction cell communication intracellular signaling cassette signal transduction phosphatidylinositol 3-kinase/protein kinase B signal transduction protein localization establishment of protein localization cellular localization nitrogen compound transport transport establishment of localization localization protein transport cellular macromolecule localization macromolecule localization T cell activation T cell differentiation regulation of localization regulation of protein localization nucleocytoplasmic transport positive regulation of nucleocytoplasmic transport nuclear transport positive regulation of biological process positive regulation of establishment of protein localization regulation of protein localization to nucleus positive regulation of protein transport import into nucleus regulation of protein import into nucleus establishment of localization in cell protein import into nucleus positive regulation of intracellular transport positive regulation of cellular process regulation of intracellular transport regulation of transport positive regulation of protein localization to nucleus regulation of establishment of protein localization positive regulation of protein import into nucleus intracellular transport regulation of nucleocytoplasmic transport protein localization to nucleus regulation of protein transport establishment of protein localization to organelle positive regulation of transport regulation of cellular localization protein localization to organelle positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport intracellular protein transport cell adhesion positive regulation of cell adhesion regulation of cell adhesion negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of biological process regulation of signal transduction negative regulation of cell communication regulation of intracellular signal transduction negative regulation of cellular process regulation of signaling negative regulation of response to stimulus regulation of MAPK cascade negative regulation of signal transduction regulation of response to stimulus MAPK cascade regulation of cell communication negative regulation of MAPK cascade cellular response to nitrogen compound response to peptide hormone insulin receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway cellular response to endogenous stimulus response to hormone enzyme-linked receptor protein signaling pathway cellular response to hormone stimulus cell surface receptor signaling pathway response to endogenous stimulus response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus response to chemical response to insulin cellular response to insulin stimulus protein localization to cell periphery protein localization to plasma membrane regulation of protein localization to cell periphery localization within membrane protein localization to membrane regulation of protein localization to plasma membrane regulation of protein localization to membrane catabolic process autophagy regulation of catabolic process regulation of autophagy metabolic process process utilizing autophagic mechanism regulation of metabolic process positive regulation of DNA-templated transcription RNA metabolic process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II positive regulation of biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process primary metabolic process regulation of macromolecule metabolic process positive regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process positive regulation of RNA metabolic process regulation of gene expression biosynthetic process positive regulation of RNA biosynthetic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of cellular component size regulation of protein-containing complex assembly protein-containing complex assembly regulation of actin filament polymerization regulation of biological quality protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization regulation of actin polymerization or depolymerization regulation of protein polymerization regulation of actin filament length regulation of anatomical structure size cellular response to stress response to stress response to endoplasmic reticulum stress	cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction intracellular anatomical structure cytoplasm cytosol protein-containing complex catalytic complex membrane phosphatidylinositol 3-kinase complex, class I membrane protein complex transferase complex cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction intracellular anatomical structure cytoplasm cytosol protein-containing complex catalytic complex membrane phosphatidylinositol 3-kinase complex, class I membrane protein complex transferase complex phosphatidylinositol 3-kinase complex, class IA phosphatidylinositol 3-kinase complex transferase complex, transferring phosphorus-containing groups extrinsic component of membrane membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR038064	Influenza A virus NS1, effector domain-like superfamily	Homologous Superfamily
A, C [auth B]	IPR009068	uS15/NS1, RNA-binding domain superfamily	Homologous Superfamily
A, C [auth B]	IPR000256	Influenza A virus NS1 protein	Family
A, C [auth B]	IPR004208	Influenza non-structural protein (NS1)	Family
B [auth C], D	IPR036860	SH2 domain superfamily	Homologous Superfamily
B [auth C], D	IPR000980	SH2 domain	Domain
B [auth C], D	IPR035586	Phosphatidylinositol 3-kinase regulatory subunit beta, SH3 domain	Domain
B [auth C], D	IPR036028	SH3-like domain superfamily	Homologous Superfamily
B [auth C], D	IPR008936	Rho GTPase activation protein	Homologous Superfamily
B [auth C], D	IPR035020	PI3K p85 subunit, C-terminal SH2 domain	Domain
B [auth C], D	IPR000198	Rho GTPase-activating protein domain	Domain
B [auth C], D	IPR032498	PI3K regulatory subunit p85-related , inter-SH2 domain	Domain
B [auth C], D	IPR001452	SH3 domain	Domain
B [auth C], D	IPR035022	PI3K p85 subunit, N-terminal SH2 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth C], D	Pharos: O00459	megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 prostate cancer cerebrovascular disorder endometrium carcinoma in situ intellectual disability Megalanecephaly Polymicrogyria-Polydactyly Hydrocephalus Syndrome polydactyly systemic sclerosis endometrial carcinoma endometrium adenocarcinoma hydrocephalus megalencephaly-polymicrogyria-postaxial polydactyly-hydrocephalus syndrome

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.