Annotations: 7D2C

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Macro	e7d2cA1	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01661	Macro domain (Macro)	Macro domain	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently ...	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently [2]; while in other cases (such as Aprataxin) it appears to bind both non-covalently through a zinc finger motif, and covalently through a separate region of the protein [3]. This domain is found in a number of otherwise unrelated proteins. It is found at the C-terminus of the macro-H2A histone protein 4 and also in the non-structural proteins of several types of ssRNA viruses such as NSP3 from alpha-viruses and coronaviruses. This domain is also found on its own in a family of proteins from bacteria, archaebacteria and eukaryotes. The 3D structure of the SARS-CoV Macro domain has a mixed alpha/beta fold consisting of a central seven-stranded twisted mixed beta sheet sandwiched between two alpha helices on one face, and three on the other. The final alpha-helix, located on the edge of the central beta-sheet, forms the C terminus of the protein [4]. The crystal structure of AF1521 (a Macro domain-only protein from Archaeoglobus fulgidus) has also been reported and compared with other Macro domain containing proteins. Several Macro domain only proteins are shorter than AF1521, and appear to lack either the first strand of the beta-sheet or the C-terminal helix 5. Well conserved residues form a hydrophobic cleft and cluster around the AF1521-ADP-ribose binding site [5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Protein mono-ADP-ribosyltransferase PARP14	pentosyltransferase activity catalytic activity, acting on a protein glycosyltransferase activity NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity transferase activity NAD+-protein mono-ADP-ribosyltransferase activity catalytic activity NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity NAD+ binding NAD binding binding nucleotide binding nucleoside phosphate binding pentosyltransferase activity catalytic activity, acting on a protein glycosyltransferase activity NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity transferase activity NAD+-protein mono-ADP-ribosyltransferase activity catalytic activity NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity NAD+ binding NAD binding binding nucleotide binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding heterocyclic compound binding small molecule binding transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups protein binding enzyme binding NAD+ poly-ADP-ribosyltransferase activity	biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism response to stress innate immune response response to external biotic stimulus cellular response to stimulus biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism response to stress innate immune response response to external biotic stimulus cellular response to stimulus regulation of cellular process negative regulation of biological process regulation of signal transduction cell communication regulation of primary metabolic process regulation of phosphate metabolic process peptidyl-amino acid modification phosphate-containing compound metabolic process negative regulation of protein modification process regulation of receptor signaling pathway via JAK-STAT signal transduction protein modification process phosphorus metabolic process negative regulation of protein metabolic process tyrosine phosphorylation of STAT protein regulation of biological process negative regulation of receptor signaling pathway via JAK-STAT protein phosphorylation phosphorylation regulation of protein metabolic process negative regulation of metabolic process primary metabolic process regulation of response to stimulus negative regulation of protein phosphorylation macromolecule modification metabolic process regulation of phosphorus metabolic process macromolecule metabolic process regulation of receptor signaling pathway via STAT regulation of metabolic process cell surface receptor signaling pathway via STAT regulation of protein modification process signaling regulation of protein phosphorylation negative regulation of signaling negative regulation of phosphate metabolic process peptidyl-tyrosine modification negative regulation of cell communication negative regulation of cellular process protein metabolic process negative regulation of macromolecule metabolic process negative regulation of peptidyl-tyrosine phosphorylation regulation of signaling cell surface receptor signaling pathway regulation of tyrosine phosphorylation of STAT protein negative regulation of response to stimulus biological regulation regulation of peptidyl-tyrosine phosphorylation regulation of macromolecule metabolic process negative regulation of signal transduction negative regulation of tyrosine phosphorylation of STAT protein negative regulation of phosphorus metabolic process regulation of cell communication cellular process regulation of phosphorylation negative regulation of phosphorylation peptidyl-tyrosine phosphorylation negative regulation of receptor signaling pathway via STAT cell surface receptor signaling pathway via JAK-STAT response to type II interferon response to cytokine regulation of type II interferon-mediated signaling pathway cellular response to type II interferon negative regulation of response to type II interferon interferon-mediated signaling pathway cellular response to cytokine stimulus regulation of immune system process regulation of innate immune response negative regulation of immune response type II interferon-mediated signaling pathway regulation of response to cytokine stimulus negative regulation of response to cytokine stimulus response to chemical negative regulation of response to external stimulus negative regulation of defense response regulation of immune response regulation of response to type II interferon regulation of defense response regulation of response to biotic stimulus regulation of response to external stimulus negative regulation of response to biotic stimulus cytokine-mediated signaling pathway regulation of response to stress negative regulation of type II interferon-mediated signaling pathway regulation of cytokine-mediated signaling pathway response to peptide negative regulation of immune system process negative regulation of cytokine-mediated signaling pathway negative regulation of innate immune response positive regulation of phosphorylation positive regulation of phosphate metabolic process positive regulation of metabolic process positive regulation of macromolecule metabolic process positive regulation of protein phosphorylation positive regulation of phosphorus metabolic process positive regulation of protein metabolic process positive regulation of biological process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein modification process positive regulation of cellular process positive regulation of tyrosine phosphorylation of STAT protein positive regulation of cell communication positive regulation of signal transduction positive regulation of response to cytokine stimulus positive regulation of response to stimulus response to interleukin-4 cellular response to interleukin-4 positive regulation of interleukin-4-mediated signaling pathway interleukin-4-mediated signaling pathway regulation of interleukin-4-mediated signaling pathway positive regulation of cytokine-mediated signaling pathway positive regulation of signaling regulation of biosynthetic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process protein poly-ADP-ribosylation post-translational protein modification	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR012317	Poly(ADP-ribose) polymerase, catalytic domain	Domain
A	IPR052056	Mono-ADP-ribosyltransferase ARTD/PARP	Family
A	IPR002589	Macro domain	Domain
A	IPR004170	WWE domain	Domain
A	IPR043472	Macro domain-like	Homologous Superfamily
A	IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	Homologous Superfamily
A	IPR037197	WWE domain superfamily	Homologous Superfamily
A	IPR034517	PARP-14, RNA recognition motif 2	Domain
A	IPR054596	Mono-ADP-ribosyltransferase PARP14, WWE domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: Q460N5	atopic eczema Crohn disease interstitial cystitis malignant glioma ovarian cancer pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor gastric carcinoma juvenile dermatomyositis malignant pancreatic neoplasm pancreatic ductal adenocarcinoma psoriasis ulcerative colitis breast cancer atopic eczema Crohn disease interstitial cystitis malignant glioma ovarian cancer pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor gastric carcinoma juvenile dermatomyositis malignant pancreatic neoplasm pancreatic ductal adenocarcinoma psoriasis ulcerative colitis breast cancer glioblastoma malignant ependymoma osteosarcoma pancreatic intraductal papillary-mucinous neoplasm Sjogren syndrome

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.