7K5J

Structure of an E1-E2-ubiquitin thioester mimetic

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth N]	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
N [auth M]	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
O	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
P	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
Q	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
R	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
W	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
X	SCOP2B Superfamily	Ubiquitin-like	8066181	3000157	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
L [auth K]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
B [auth D]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
D [auth A]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
J [auth I]	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
S	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	E2 binding domain-like	8003942	3000147	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	FCCH domain-like	8003532	3000138	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	CC (catalytic cysteine) domain-like	8003552	3000140	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8017546	3000112	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	Activating enzymes of the ubiquitin-like proteins	8003540	3000112	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	SCCH domain-like	8003543	3000139	SCOP2B (2022-06-29)
E [auth B]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
C [auth F]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
G [auth E]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
I [auth H]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
K [auth J]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
M [auth L]	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
T	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)
V	SCOP2B Superfamily	UBC-like	8095691	3000570	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth N]	ubiquitin_1	e7k5jN1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
N [auth M]	ubiquitin_1	e7k5jM1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
O	ubiquitin_1	e7k5jO1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
P	ubiquitin_1	e7k5jP1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
Q	ubiquitin_1	e7k5jQ1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
R	ubiquitin_1	e7k5jR1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
W	ubiquitin_1	e7k5jW1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
X	ubiquitin_1	e7k5jX1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
F [auth C]	E1_FCCH	e7k5jC2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
F [auth C]	UBA_e1_thiolCys	e7k5jC5	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
F [auth C]	ThiF_3	e7k5jC1	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
F [auth C]	E1_UFD	e7k5jC6	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
F [auth C]	ThiF_1	e7k5jC3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
F [auth C]	ThiF_3	e7k5jC4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
L [auth K]	E1_FCCH	e7k5jK1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
L [auth K]	UBA_e1_thiolCys	e7k5jK5	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
L [auth K]	ThiF_3	e7k5jK6	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
L [auth K]	E1_UFD	e7k5jK4	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
L [auth K]	ThiF_1	e7k5jK3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
L [auth K]	ThiF_3	e7k5jK2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
B [auth D]	E1_FCCH	e7k5jD6	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
B [auth D]	UBA_e1_thiolCys	e7k5jD1	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
B [auth D]	ThiF_3	e7k5jD5	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
B [auth D]	E1_UFD	e7k5jD3	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
B [auth D]	ThiF_1	e7k5jD2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
B [auth D]	ThiF_3	e7k5jD4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
D [auth A]	E1_FCCH	e7k5jA6	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
D [auth A]	UBA_e1_thiolCys	e7k5jA3	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
D [auth A]	ThiF_3	e7k5jA4	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
D [auth A]	E1_UFD	e7k5jA1	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
D [auth A]	ThiF_1	e7k5jA2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
D [auth A]	ThiF_3	e7k5jA5	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
H [auth G]	E1_FCCH	e7k5jG1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
H [auth G]	UBA_e1_thiolCys	e7k5jG4	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
H [auth G]	ThiF_3	e7k5jG2	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
H [auth G]	E1_UFD	e7k5jG3	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
H [auth G]	ThiF_1	e7k5jG6	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
H [auth G]	ThiF_3	e7k5jG5	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
J [auth I]	E1_FCCH	e7k5jI6	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
J [auth I]	UBA_e1_thiolCys	e7k5jI3	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
J [auth I]	ThiF_3	e7k5jI4	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
J [auth I]	E1_UFD	e7k5jI2	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
J [auth I]	ThiF_1	e7k5jI1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
J [auth I]	ThiF_3	e7k5jI5	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
S	E1_FCCH	e7k5jS5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
S	UBA_e1_thiolCys	e7k5jS3	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
S	ThiF_3	e7k5jS1	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
S	E1_UFD	e7k5jS6	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
S	ThiF_1	e7k5jS2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
S	ThiF_3	e7k5jS4	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
U	E1_FCCH	e7k5jU3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: E1_FCCH	ECOD (1.6)
U	UBA_e1_thiolCys	e7k5jU5	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: UBA_e1_thiolCys	ECOD (1.6)
U	ThiF_3	e7k5jU2	A: alpha arrays	X: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	H: Catalytic cysteine domain in ubiquitin-activating enzyme (From Topology)	T: Catalytic cysteine domain in ubiquitin-activating enzyme	F: ThiF_3	ECOD (1.6)
U	E1_UFD	e7k5jU4	A: a+b two layers	X: beta-Grasp	H: E2-binding domain of E1 (From Topology)	T: E2-binding domain of E1	F: E1_UFD	ECOD (1.6)
U	ThiF_1	e7k5jU6	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_1	ECOD (1.6)
U	ThiF_3	e7k5jU1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: Activating enzymes of the ubiquitin-like proteins	F: ThiF_3	ECOD (1.6)
E [auth B]	UQ_con	e7k5jB1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
C [auth F]	UQ_con	e7k5jF1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
G [auth E]	UQ_con	e7k5jE1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
I [auth H]	UQ_con	e7k5jH1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
K [auth J]	UQ_con	e7k5jJ1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
M [auth L]	UQ_con	e7k5jL1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
T	UQ_con	e7k5jT1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)
V	UQ_con	e7k5jV1	A: a+b two layers	X: UBC-like	H: UBC-like (From Topology)	T: UBC-like	F: UQ_con	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	PF00240	Ubiquitin family (ubiquitin)	Ubiquitin family	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry ...	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry a distinctive five-residue motif termed the proteasome-interacting motif (PIM), which may have a biologically significant role in protein delivery to proteasomes and recruitment of proteasomes to transcription sites [5].	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	PF00899	ThiF family (ThiF)	ThiF family	This domain is found in ubiquitin activating E1 family and members of the bacterial ThiF/MoeB/HesA family. It is repeated in Ubiquitin-activating enzyme E1 [1-3].	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	PF09358	Ubiquitin fold domain (E1_UFD)	Ubiquitin fold domain	The ubiquitin fold domain is found at the C-terminus of ubiquitin-activating E1 family enzymes. This domain binds to E2 enzymes [1].	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	PF10585	Ubiquitin-activating enzyme, SCCH domain (UBA_E1_SCCH)	Ubiquitin-activating enzyme, SCCH domain	Ubiquitin-activating enzyme (E1 enzyme) activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free A ...	Ubiquitin-activating enzyme (E1 enzyme) activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. Later the ubiquitin moiety is transferred to a cysteine residue on one of the many forms of ubiquitin-conjugating enzymes (E2) [1]. This domain carries the last of five conserved cysteines that is part of the active site of the enzyme, responsible for ubiquitin thiolester complex formation, the active site being represented by the sequence motif PICTLKNFP [2,3,4]. The catalytic cysteine domain contains the E1 active site cysteine, and is divided in two half-domains, FCCH and SCCH, for 'first' and 'second' catalytic cysteine half-domain, respectively. This is the SCCH domain in which resides the catalytic cysteine [5].	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	PF16190	Ubiquitin-activating enzyme E1 FCCH domain (E1_FCCH)	Ubiquitin-activating enzyme E1 FCCH domain	This domain is found in the ubiquitin-activating E1 family enzymes [1].	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	PF16191	Ubiquitin-activating enzyme E1 four-helix bundle (E1_4HB)	Ubiquitin-activating enzyme E1 four-helix bundle	This domain is found in the ubiquitin-activating E1 family enzymes [1].	Domain
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	PF00179	Ubiquitin-conjugating enzyme (UQ_con)	Ubiquitin-conjugating enzyme	Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. TSG101 is one of several UBC homologues that lacks this active site cystein ...	Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. TSG101 is one of several UBC homologues that lacks this active site cysteine [4, 5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	Ubiquitin	RNA binding binding organic cyclic compound binding nucleic acid binding mRNA binding	-	cellular anatomical structure intracellular anatomical structure cytoplasm membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	Ubiquitin-activating enzyme E1 1	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ubiquitin-like modifier activating enzyme activity ligase activity, forming carbon-sulfur bonds catalytic activity, acting on a protein ubiquitin activating enzyme activity catalytic activity ligase activity ATP-dependent activity	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal	cellular anatomical structure intracellular anatomical structure cytoplasm membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	Ubiquitin-conjugating enzyme E2-34 kDa	ubiquitin conjugating enzyme activity aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein conjugating enzyme activity transferase activity catalytic activity ubiquitin-protein transferase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding ubiquitin conjugating enzyme activity aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein conjugating enzyme activity transferase activity catalytic activity ubiquitin-protein transferase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding	nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of mitotic cell cycle regulation of biological process regulation of cellular process biological regulation cell cycle regulation of cell cycle nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of mitotic cell cycle regulation of biological process regulation of cellular process biological regulation cell cycle regulation of cell cycle mitotic cell cycle organelle fusion cellular component organization organelle organization mitochondrial fusion cellular component organization or biogenesis mitochondrion organization cell division protein autoubiquitination protein ubiquitination macromolecule modification protein metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal negative regulation of biological process chromatin organization negative regulation of cellular process chromatin remodeling negative regulation of macromolecule metabolic process regulation of biosynthetic process heterochromatin formation epigenetic regulation of gene expression negative regulation of metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression constitutive heterochromatin formation biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process silent mating-type cassette heterochromatin formation negative regulation of gene expression, epigenetic regulation of macromolecule biosynthetic process regulation of metabolic process catabolic process proteolysis involved in protein catabolic process proteasomal protein catabolic process protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process oxoacid metabolic process sulfur amino acid metabolic process carboxylic acid metabolic process organic acid metabolic process regulation of sulfur metabolic process regulation of small molecule metabolic process sulfur compound metabolic process small molecule metabolic process regulation of sulfur amino acid metabolic process protein polyubiquitination regulation of localization hexose transmembrane transport regulation of transport transport positive regulation of biological process carbohydrate transport establishment of localization positive regulation of D-glucose transmembrane transport transmembrane transport localization regulation of transmembrane transport positive regulation of transport D-glucose transmembrane transport positive regulation of transmembrane transport regulation of D-glucose transmembrane transport monosaccharide transmembrane transport carbohydrate transmembrane transport positive regulation of cellular process subtelomeric heterochromatin formation telomere organization chromosome organization RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by galactose regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process regulation of DNA-templated transcription mitotic cell cycle process cell cycle phase transition G1/S transition of mitotic cell cycle cell cycle G1/S phase transition mitotic cell cycle phase transition cell cycle process response to stimulus response to chemical cellular response to methylmercury response to methylmercury cellular response to stimulus DNA damage checkpoint signaling regulation of cell cycle phase transition signaling negative regulation of cell cycle intracellular signal transduction negative regulation of mitotic cell cycle cell communication mitotic DNA integrity checkpoint signaling cellular response to stress response to stress mitotic DNA damage checkpoint signaling negative regulation of cell cycle phase transition signal transduction mitotic intra-S DNA damage checkpoint signaling regulation of cell cycle process DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling DNA damage response signal transduction in response to DNA damage cell cycle G2/M phase transition G2/M transition of mitotic cell cycle	cellular anatomical structure intracellular anatomical structure cytoplasm cullin-RING ubiquitin ligase complex protein-containing complex ubiquitin ligase complex catalytic complex SCF ubiquitin ligase complex transferase complex intracellular protein-containing complex intracellular organelle organelle membraneless organelle intracellular membraneless organelle cellular anatomical structure intracellular anatomical structure cytoplasm cullin-RING ubiquitin ligase complex protein-containing complex ubiquitin ligase complex catalytic complex SCF ubiquitin ligase complex transferase complex intracellular protein-containing complex intracellular organelle organelle membraneless organelle intracellular membraneless organelle chromosome chromosome, telomeric region chromosomal region membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	IPR019956	Ubiquitin domain	Domain
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	IPR019954	Ubiquitin conserved site	Conserved Site
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	IPR050158	Ubiquitin and ubiquitin-like	Family
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
A [auth N], N [auth M], O, P, Q, A [auth N], N [auth M], O, P, Q, R, W, X	IPR000626	Ubiquitin-like domain	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR042302	Ubiquitin-activating enzyme E1, FCCH domain superfamily	Homologous Superfamily
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR000011	Ubiquitin/SUMO-activating enzyme E1-like	Family
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR045886	ThiF/MoeB/HesA family	Family
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR042449	Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1	Homologous Superfamily
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR019572	Ubiquitin-activating enzyme, SCCH domain	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR042063	Ubiquitin-activating enzyme E1, SCCH domain	Homologous Superfamily
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR000594	THIF-type NAD/FAD binding fold	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR032420	Ubiquitin-activating enzyme E1, four-helix bundle	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR018075	Ubiquitin-activating enzyme E1	Family
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR032418	Ubiquitin-activating enzyme E1, FCCH domain	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR018965	Ubiquitin-activating enzyme E1, C-terminal	Domain
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR035985	Ubiquitin-activating enzyme	Homologous Superfamily
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR033127	Ubiquitin-activating enzyme E1, Cys active site	Active Site
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR038252	Ubiquitin-activating enzyme E1, C-terminal domain superfamily	Homologous Superfamily
B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], B [auth D], D [auth A], F [auth C], H [auth G], J [auth I], L [auth K], S, U	IPR018074	Ubiquitin-activating enzyme E1, conserved site	Conserved Site
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	IPR050113	Ubiquitin-conjugating enzyme	Family
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	IPR016135	Ubiquitin-conjugating enzyme/RWD-like	Homologous Superfamily
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	IPR000608	Ubiquitin-conjugating enzyme E2	Domain
C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], C [auth F], E [auth B], G [auth E], I [auth H], K [auth J], M [auth L], T, V	IPR023313	Ubiquitin-conjugating enzyme, active site	Active Site