pH 6.8, polymerization of the microtubule, temperature 296K
Sample
KIF1A HEAD DECORATED MICROTUBULE
Specimen Preparation
Sample Aggregation State
FILAMENT
Vitrification Instrument
Cryogen Name
Sample Vitrification Details
PLUNGED INTO ETHANE
3D Reconstruction
Reconstruction Method
HELICAL
Number of Particles
37000
Reported Resolution (Å)
15
Resolution Method
Other Details
THREE-DIMENSIONAL RECONSTRUCTION METHOD:
FOURIER-BESSEL-TRANSFORM (DEROSIER AND MOORE, 1970
J.MOL.BIOL ...
THREE-DIMENSIONAL RECONSTRUCTION METHOD:
FOURIER-BESSEL-TRANSFORM (DEROSIER AND MOORE, 1970
J.MOL.BIOL. 52, 355-369; BEROUKHIM AND UNWIN, 1997
ULTRAMICROSCOPY 70, 57-81).
Software used: MRC PROGRAMS.
EFFECTIVE RESOLUTION OF THE RECONSTRUCTION:
THE RESOLUTION OF THE FINAL RECONSTRUCTED DENSITY WAS
DETERMINED TO BE AT LEAST 15 ANGSTROMS, AS MEASURED BY
RANDOMLY SPLITTING THE PARTICLES INTO TWO SETS AND
CALCULATING THE FOURIER SHELL CORRELATIONS BETWEEN THE
TWO INDEPENDENT SET. (HEEL, 1987, ULTRAMICROSCOPY 21,
95-100).
1IA0 is an atomic model of KIF1A head-microtubule complex, which contains
KIF1A, alpha-tubulin, and beta-tubulin. Since alpha- and beta-tubulin
were indistinguishable at 15A resolution, the assignment of alpha- and
beta-tubulin was arbitrary when 1IA0 was deposited. Authors state that they were aware of the
limitation and didn't mention about the assignment of alpha- and
beta-tubulin in the relevant 2001 Nature paper. Therefore, the main conclusions
in the paper are still hold true. However, subsequently the assignment of alpha- and beta-tubulin turned
out to be wrong and should be swapped. For the correct assignment
and better structures, please use 2HXF. This is based on higher
resolution (10 ) structure, which was published in EMBO J. 2006.
25:4187-4194.
