2K88 | pdb_00002k88

Association of subunit d (Vma6p) and E (Vma4p) with G (Vma10p) and the NMR solution structure of subunit G (G1-59) of the Saccharomyces cerevisiae V1VO ATPase

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-15N HSQC	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
2	2D 1H-13C HSQC	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
3	3D CBCA(CO)NH	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
4	3D HNCACB	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
5	3D HNCO	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
6	3D H(CCO)NH	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
7	3D C(CO)NH	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
8	3D 1H-15N NOESY	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288
9	3D 1H-13C NOESY	25 mM sodium phosphate	90% H2O/10% D2O	0	6.8	AMBIENT	288

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	600

NMR Refinement
Method	Details	Software
torsion angle dynamics		CYANA

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	10
Conformers Submitted Total Number	10
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	CYANA	3.0	GUNTERT, P. ET AL.
2	collection	TopSpin		Bruker Biospin
3	data analysis	Sparky		Goddard, T. et al.
4	data analysis	MOLMOL		Koradi, R. et al.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.