2LOM | pdb_00002lom

Backbone structure of human membrane protein HIGD1A


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D 1H-15N HSQC13C(1),15N-Selectively labeled-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
22D HNCO13C(1),15N-Selectively labeled-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
33D HNCO13C,15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
43D HNCA13C,15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
53D HNCACB13C,15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
63D 1H-15N NOESY15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
73D 13C-15N HSQC-NOESY-HSQC13C,15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
82D 1H-15N HSQC15N-HIGD1A, 20 mM MES-Bis-TRIS, 2 % LMPG95% H2O/5% D2O406.0ambient310
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX700
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsCYANA
NMR Ensemble Information
Conformer Selection Criteriatarget function
Conformers Calculated Total Number200
Conformers Submitted Total Number20
Representative Model1 (fewest violations)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionCYANA1.04Guntert, Mumenthaler and Wuthrich
2collectionTopSpinBruker Biospin
3combinatorial chemical shift assignmentMCCL1.0(MCCL) Kwiatkowski, Maslennikov
4data analysisMCCL1.0(MCCL) Kwiatkowski, Maslennikov
5chemical shift assignmentCARAKeller and Wuthrich
6data analysisCARAKeller and Wuthrich
7refinementCYANAGuntert, Mumenthaler and Wuthrich