Download MendeleyC-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity
Umetsu, Y., Aizawa, T., Muto, K., Yamamoto, H., Kamiya, M., Kumaki, Y., Mizuguchi, M., Demura, M., Hayakawa, Y., Kawano, K.(2009) J Biological Chem 284: 29625-29634
- PubMed: 19710009
- DOI: https://doi.org/10.1074/jbc.M109.011148
- Primary Citation of Related Structures:
2EQH, 2EQQ, 2EQT - PubMed Abstract:
Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.
Organizational Affiliation:
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
