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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.

Weierstall, U., James, D., Wang, C., White, T.A., Wang, D., Liu, W., Spence, J.C., Bruce Doak, R., Nelson, G., Fromme, P., Fromme, R., Grotjohann, I., Kupitz, C., Zatsepin, N.A., Liu, H., Basu, S., Wacker, D., Han, G.W., Katritch, V., Boutet, S., Messerschmidt, M., Williams, G.J., Koglin, J.E., Marvin Seibert, M., Klinker, M., Gati, C., Shoeman, R.L., Barty, A., Chapman, H.N., Kirian, R.A., Beyerlein, K.R., Stevens, R.C., Li, D., Shah, S.T., Howe, N., Caffrey, M., Cherezov, V.

(2014) Nat Commun 5: 3309-3309

• PubMed: 24525480 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1038/ncomms4309
• Primary Citation of Related Structures:  
  4O9R


• PubMed Abstract: 
  

  Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

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Organizational Affiliation: 

Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.