Download MendeleySubstrate Recognition, Directional Flux, and Allostery in a Synaptic Vesicle Glutamate Transporter
Li, F., Stroud, R.M.To be published.
7T3N | pdb_00007t3n
R184Q/E191Q mutant of rat vesicular glutamate transporter 2 (VGLUT2)
- PDB DOI: https://doi.org/10.2210/pdb7T3N/pdb
- EM Map EMD-25665: EMDB EMDataResource
- Classification: TRANSPORT PROTEIN
- Organism(s): Rattus norvegicus
- Expression System: Spodoptera frugiperda
- Mutation(s): Yes
- Membrane Protein: Yes OPMPDBTMMemProtMD
- Deposited: 2021-12-08 Released: 2022-12-14
- Funding Organization(s): National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS), National Institutes of Health/National Institute of Mental Health (NIH/NIMH)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 3.00 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Vesicular glutamate transporter 2 | 582 | Rattus norvegicus | Mutation(s): 2 Gene Names: Slc17a6, Dnpi, Vglut2 Membrane Entity: Yes |  | |
UniProt | |||||
Find proteins for Q9JI12 (Rattus norvegicus) Explore Q9JI12 Go to UniProtKB: Q9JI12 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9JI12 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| I7Y Query on I7Y | C [auth A] | (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside C45 H74 O15 MUCLRRLHERFRKG-ZHIGRBBGSA-N |  | ||
| C5B (Subject of Investigation/LOI) Query on C5B | B [auth A] | (1S,3R)-1-AMINOCYCLOPENTANE-1,3-DICARBOXYLIC ACID C7 H11 N O4 YFYNOWXBIBKGHB-FBCQKBJTSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| P1L Query on P1L | A | L-PEPTIDE LINKING | C19 H37 N O3 S |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 3.00 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| MODEL REFINEMENT | PHENIX |
Entry History & Funding Information
Deposition Data
- Released Date: 2022-12-14 Deposition Author(s): Li, F., Stroud, R.M., Edwards, R.H.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) | United States | R01NS089713 |
| National Institutes of Health/National Institute of Mental Health (NIH/NIMH) | United States | K99MH119591 |
Revision History (Full details and data files)
- Version 1.0: 2022-12-14
Type: Initial release
