8IKU | pdb_00008iku

Aldo-keto reductase KmAKR - W297H

PDB DOI: https://doi.org/10.2210/pdb8IKU/pdb

Classification: OXIDOREDUCTASE
Organism(s): Kluyveromyces marxianus
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2023-03-01 Released: 2024-03-06
Deposition Author(s): Xu, S.Y., Zhou, L., Xu, Y., Wang, Y.J., Zheng, Y.G.
Funding Organization(s): National Natural Science Foundation of China (NSFC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.13 Å
R-Value Free:
0.260 (Depositor), 0.260 (DCC)
R-Value Work:
0.212 (Depositor), 0.220 (DCC)
R-Value Observed:
0.215 (Depositor)

Starting Model: in silico
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wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Aldo-keto reductase KmAKR - W297H from Kluyveromyces marxianus

Xu, S.Y., Zhou, L., Xu, Y., Wang, Y.J., Zheng, Y.G.

To be published.

Macromolecule Content

Total Structure Weight: 35.38 kDa
Atom Count: 2,503
Modelled Residue Count: 307
Deposited Residue Count: 310
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
NADPH-dependent alpha-keto amide reductase	A	310	Kluyveromyces marxianus	Mutation(s): 1 Gene Names: KLMA_60481
UniProt
Find proteins for W0TDP7 (Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275)) Explore W0TDP7 Go to UniProtKB: W0TDP7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	W0TDP7
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.13 Å
R-Value Free: 0.260 (Depositor), 0.260 (DCC)
R-Value Work: 0.212 (Depositor), 0.220 (DCC)
R-Value Observed: 0.215 (Depositor)

Space Group: C 1 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 97.453	α = 90
b = 48.031	β = 104.01
c = 66.468	γ = 90

Software Package:

Software Name	Purpose
DIALS	data scaling
REFMAC	refinement
DIALS	data reduction
MOLREP	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2024-03-06

Deposition Author(s):

Funding Organization	Location	Grant Number
National Natural Science Foundation of China (NSFC)	China	22178318

Revision History (Full details and data files)

Version 1.0: 2024-03-06
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.