1F3U | pdb_00001f3u

CRYSTAL STRUCTURE OF THE RAP30/74 INTERACTION DOMAINS OF HUMAN TFIIF

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1f3ua_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF beta subunit, Rap30	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d1f3uc_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF beta subunit, Rap30	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E	d1f3ue_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF beta subunit, Rap30	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
G	d1f3ug_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF beta subunit, Rap30	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1f3ub_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF alpha subunit, Rap74	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d1f3ud_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF alpha subunit, Rap74	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F	d1f3uf_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF alpha subunit, Rap74	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
H	d1f3uh_	All beta proteins	triple barrel	Rap30/74 interaction domain-like	Rap30/74 interaction domains	TFIIF alpha subunit, Rap74	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	TFIIF beta subunit, Rap30	8054047	4007626	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Rap30/74 heterodimerisation domain-like	8003550	3000142	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003550	3000142	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003550	3000142	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003550	3000142	SCOP2B (2022-06-29)
B	SCOP2 Family	TFIIF alpha subunit, Rap74	8054048	4007627	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Rap30/74 heterodimerisation domain-like	8003556	3000142	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003556	3000142	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003556	3000142	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Rap30/74 heterodimerisation domain-like	8003556	3000142	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	TFIIF_beta_N_1	e1f3uA1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_beta_N_1	ECOD (1.6)
C	TFIIF_beta_N_1	e1f3uC1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_beta_N_1	ECOD (1.6)
E	TFIIF_beta_N_1	e1f3uE1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_beta_N_1	ECOD (1.6)
G	TFIIF_beta_N_1	e1f3uG1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_beta_N_1	ECOD (1.6)
B	TFIIF_beta_N_1	e1f3uB1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_beta_N_1	ECOD (1.6)
D	TFIIF_alpha_N	e1f3uD1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_alpha_N	ECOD (1.6)
F	TFIIF_alpha_N	e1f3uF1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_alpha_N	ECOD (1.6)
H	TFIIF_alpha_N	e1f3uH1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: Rap30/74 interaction domains-like	F: TFIIF_alpha_N	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C, E, G	PF17683	TFIIF, beta subunit N-terminus (TFIIF_beta_N)	TFIIF, beta subunit N-terminus	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G	TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT	DNA binding binding nucleic acid binding nucleic acid conformation isomerase activity isomerase activity DNA helicase activity catalytic activity, acting on a nucleic acid single-stranded DNA helicase activity helicase activity 3'-5' DNA helicase activity ATP-dependent activity ATP-dependent activity, acting on DNA catalytic activity, acting on DNA single-stranded 3'-5' DNA helicase activity DNA binding binding nucleic acid binding nucleic acid conformation isomerase activity isomerase activity DNA helicase activity catalytic activity, acting on a nucleic acid single-stranded DNA helicase activity helicase activity 3'-5' DNA helicase activity ATP-dependent activity ATP-dependent activity, acting on DNA catalytic activity, acting on DNA single-stranded 3'-5' DNA helicase activity catalytic activity macromolecular conformation isomerase activity four-way junction helicase activity general transcription initiation factor activity RNA polymerase II general transcription initiation factor activity forked DNA-dependent helicase activity double-stranded DNA helicase activity	RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process DNA-templated transcription initiation RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription by RNA polymerase II biosynthetic process metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process DNA-templated transcription initiation RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription by RNA polymerase II biosynthetic process metabolic process cellular process macromolecule metabolic process transcription initiation at RNA polymerase II promoter transcription elongation by RNA polymerase II DNA-templated transcription elongation regulation of cellular process positive regulation of DNA-templated transcription positive regulation of biological process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process positive regulation of metabolic process regulation of RNA metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II regulation of biological process positive regulation of nucleobase-containing compound metabolic process biological regulation regulation of macromolecule metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process regulation of gene expression positive regulation of RNA biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process	RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle cellular anatomical structure nucleoplasm RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle cellular anatomical structure nucleoplasm catalytic complex intracellular membrane-bounded organelle transcription factor TFIIF complex transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen cytoskeleton membraneless organelle intracellular membraneless organelle microtubule cytoskeleton
B, D, F, H	TRANSCRIPTION INITIATION FACTOR IIF, ALPHA SUBUNIT	protein binding binding protein domain specific binding transcription factor binding basal transcription machinery binding basal RNA polymerase II transcription machinery binding general transcription initiation factor binding RNA polymerase II general transcription initiation factor binding RNA binding nucleic acid binding chromatin binding promoter-specific chromatin binding protein phosphatase binding phosphatase binding protein binding binding protein domain specific binding transcription factor binding basal transcription machinery binding basal RNA polymerase II transcription machinery binding general transcription initiation factor binding RNA polymerase II general transcription initiation factor binding RNA binding nucleic acid binding chromatin binding promoter-specific chromatin binding protein phosphatase binding phosphatase binding enzyme binding DNA binding general transcription initiation factor activity RNA polymerase II general transcription initiation factor activity phosphoric ester hydrolase activity enzyme regulator activity phosphatase activator activity molecular function activator activity phosphatase regulator activity phosphatase activity hydrolase activity enzyme activator activity hydrolase activity, acting on ester bonds molecular function regulator activity catalytic activity TFIIF-class transcription factor complex binding protein-containing complex binding	regulation of protein binding regulation of molecular function negative regulation of protein binding regulation of binding negative regulation of binding biological regulation negative regulation of molecular function RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process regulation of protein binding regulation of molecular function negative regulation of protein binding regulation of binding negative regulation of binding biological regulation negative regulation of molecular function RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription by RNA polymerase II biosynthetic process metabolic process cellular process macromolecule metabolic process DNA-templated transcription initiation transcription initiation at RNA polymerase II promoter biological process involved in interspecies interaction between organisms response to stimulus response to other organism response to external stimulus response to external biotic stimulus response to virus response to biotic stimulus transcription elongation by RNA polymerase II DNA-templated transcription elongation regulation of cellular process positive regulation of DNA-templated transcription positive regulation of transcription elongation by RNA polymerase II positive regulation of biological process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process positive regulation of metabolic process regulation of RNA metabolic process positive regulation of biosynthetic process positive regulation of DNA-templated transcription, elongation positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II regulation of DNA-templated transcription elongation regulation of biological process positive regulation of nucleobase-containing compound metabolic process regulation of transcription elongation by RNA polymerase II regulation of macromolecule metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process regulation of gene expression positive regulation of RNA biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process	cellular anatomical structure cell junction RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle cellular anatomical structure cell junction RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle transcription factor TFIIF complex transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen transcription factor TFIID complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C, E, G	IPR003196	Transcription initiation factor IIF, beta subunit	Family
A, C, E, G	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, C, E, G	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, C, E, G	IPR040450	TFIIF beta subunit, HTH domain	Domain
A, C, E, G	IPR040504	TFIIF, beta subunit, N-terminal	Domain
A, C, E, G	IPR011039	Transcription Factor IIF, Rap30/Rap74, interaction	Homologous Superfamily
B, D, F, H	IPR008851	Transcription initiation factor IIF, alpha subunit	Family
B, D, F, H	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
B, D, F, H	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
B, D, F, H	IPR011039	Transcription Factor IIF, Rap30/Rap74, interaction	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, C, E, G	Pharos: P13984	lung cancer osteosarcoma psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.