1NDH | pdb_00001ndh

CRYSTAL STRUCTURE OF NADH-CYTOCHROME B5 REDUCTASE FROM PIG LIVER AT 2.4 ANGSTROMS RESOLUTION

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1ndha1	All beta proteins	Reductase/isomerase/elongation factor common domain	Riboflavin synthase domain-like	Ferredoxin reductase FAD-binding domain-like	cytochrome b5 reductase	pig (Sus scrofa ) [TaxId: 9823 ],	SCOPe (2.08)
A	d1ndha2	Alpha and beta proteins (a/b)	Ferredoxin reductase-like, C-terminal NADP-linked domain	Ferredoxin reductase-like, C-terminal NADP-linked domain	Reductases	cytochrome b5 reductase	pig (Sus scrofa ) [TaxId: 9823 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Ferredoxin reductase FAD-binding domain-like	8029678	4002840	SCOP2 (2022-06-29)
A	SCOP2 Family	Reductases	8031816	4003770	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Riboflavin synthase domain-like	8042057	3001812	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Ferredoxin reductase-like, C-terminal NADP-linked domain	8044194	3001461	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FAD_binding_6	e1ndhA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: FAD_binding_6	ECOD (1.6)
A	NAD_binding_1	e1ndhA2	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: NAD_binding_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
A	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00175	Oxidoreductase NAD-binding domain (NAD_binding_1)	Oxidoreductase NAD-binding domain	Xanthine dehydrogenases, that also bind FAD/NAD, have essentially no similarity.	Domain
A	PF00970	Oxidoreductase FAD-binding domain (FAD_binding_6)	Oxidoreductase FAD-binding domain		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	CYTOCHROME B5 REDUCTASE	FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity cytochrome-b5 reductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor catalytic activity	secondary alcohol biosynthetic process small molecule biosynthetic process cholesterol biosynthetic process sterol metabolic process steroid metabolic process cholesterol metabolic process lipid biosynthetic process alcohol metabolic process primary metabolic process secondary alcohol metabolic process biosynthetic process sterol biosynthetic process alcohol biosynthetic process small molecule metabolic process secondary alcohol biosynthetic process small molecule biosynthetic process cholesterol biosynthetic process sterol metabolic process steroid metabolic process cholesterol metabolic process lipid biosynthetic process alcohol metabolic process primary metabolic process secondary alcohol metabolic process biosynthetic process sterol biosynthetic process alcohol biosynthetic process small molecule metabolic process metabolic process cellular process steroid biosynthetic process lipid metabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope mitochondrial outer membrane outer membrane organelle membrane organelle subcompartment endomembrane system endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	Domain
A	IPR001433	Oxidoreductase FAD/NAD(P)-binding	Domain
A	IPR001834	NADH:cytochrome b5 reductase-like	Family
A	IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	Homologous Superfamily
A	IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	Domain
A	IPR017938	Riboflavin synthase-like beta-barrel	Homologous Superfamily
A	IPR017927	FAD-binding domain, ferredoxin reductase-type	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	cytochrome-b5 reductase M-CSA #862	NADH:cytochrome b5 reductase (CBR) serves as electron donor for cytochrome b5, a ubiquitous electron carrier, thus participating in a variety of metabolic pathways (including steroid biosynthesis, desaturation and elongation of fatty acids, P450-dependent reactions, methaemoglobin reduction, etc.).	Defined by 4 residues: HIS:A-49TYR:A-65THR:A-66CYS:A-245 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 1.6.2.2 (PDB Primary Data) Search M-CSA Motif + EC 1.6.2.2

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.