Annotations: 5B48

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	POR	e5b48A3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
A	Transketolase_C	e5b48A2	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
A	POR_N	e5b48A1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
C	POR	e5b48C2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: Pyruvate-ferredoxin oxidoreductase, PFOR, domain III	F: POR	ECOD (1.6)
C	Transketolase_C	e5b48C3	A: a/b three-layered sandwiches	X: TK C-terminal domain-like (From Topology)	H: TK C-terminal domain-like (From Topology)	T: TK C-terminal domain-like	F: Transketolase_C	ECOD (1.6)
C	POR_N	e5b48C1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: POR_N	ECOD (1.6)
B	TPP_enzyme_C	e5b48B1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)
D	TPP_enzyme_C	e5b48D1	A: a/b three-layered sandwiches	X: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	H: Thiamin diphosphate-binding fold (THDP-binding) (From Topology)	T: Thiamin diphosphate-binding fold (THDP-binding)	F: TPP_enzyme_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.970	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains	CATH (utative)
A	3.40.50.920	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (utative)
C	3.40.50.970	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains	CATH (utative)
C	3.40.50.920	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF01558	Pyruvate ferredoxin/flavodoxin oxidoreductase (POR)	Pyruvate ferredoxin/flavodoxin oxidoreductase	This family includes a region of the large protein pyruvate-flavodoxin oxidoreductase and the whole pyruvate ferredoxin oxidoreductase gamma subunit protein. It is not known whether the gamma subunit has a catalytic or regulatory role. Pyruvate oxi ...	This family includes a region of the large protein pyruvate-flavodoxin oxidoreductase and the whole pyruvate ferredoxin oxidoreductase gamma subunit protein. It is not known whether the gamma subunit has a catalytic or regulatory role. Pyruvate oxidoreductase (POR) catalyses the final step in the fermentation of carbohydrates in anaerobic microorganisms [1]. This involves the oxidative decarboxylation of pyruvate with the participation of thiamine followed by the transfer of an acetyl moiety to coenzyme A for the synthesis of acetyl-CoA [1]. The family also includes pyruvate flavodoxin oxidoreductase as encoded by the nifJ gene in cyanobacterium which is required for growth on molecular nitrogen when iron is limited [2].	Domain
A, C	PF01855	Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (POR_N)	Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg	-	Family
B, D	PF12367	Pyruvate ferredoxin oxidoreductase beta subunit C terminal (PFO_beta_C)	Pyruvate ferredoxin oxidoreductase beta subunit C terminal	-	Family
B, D	PF02775	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (TPP_enzyme_C)	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	2-oxoacid--ferredoxin oxidoreductase alpha subunit	oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors 2-oxobutyrate synthase activity catalytic activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor pyruvate synthase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor 2-oxoglutarate synthase activity	response to oxidative stress response to stimulus response to stress	-
B, D	2-oxoacid--ferredoxin oxidoreductase beta subunit	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors 2-oxobutyrate synthase activity catalytic activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor pyruvate synthase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor 2-oxoglutarate synthase activity cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors 2-oxobutyrate synthase activity catalytic activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor pyruvate synthase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor 2-oxoglutarate synthase activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding vitamin binding thiamine pyrophosphate binding anion binding quaternary ammonium group binding heterocyclic compound binding sulfur compound binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR002869	Pyruvate-flavodoxin oxidoreductase, central domain	Homologous Superfamily
A, C	IPR053400	2-oxoacid:ferredoxin oxidoreductase alpha	Family
A, C	IPR022367	2-oxoacid:acceptor oxidoreductase, alpha subunit	Family
A, C	IPR029061	Thiamin diphosphate-binding fold	Homologous Superfamily
A, C	IPR009014	Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II	Homologous Superfamily
A, C	IPR019752	Pyruvate/ketoisovalerate oxidoreductase, catalytic domain	Domain
A, C	IPR002880	Pyruvate flavodoxin/ferredoxin oxidoreductase, pyrimidine binding domain	Domain
A, C	IPR050722	Pyruvate:ferredoxin/flavodoxin oxidoreductase	Family
B, D	IPR053399	2-oxoacid:ferredoxin oxidoreductase beta subunit	Family
B, D	IPR051457	2-oxoacid:ferredoxin oxidoreductase	Family
B, D	IPR032686	Pyruvate ferredoxin oxidoreductase beta subunit, C-terminal	Domain
B, D	IPR029061	Thiamin diphosphate-binding fold	Homologous Superfamily
B, D	IPR011896	2-oxoacid:acceptor oxidoreductase, beta subunit	Family
B, D	IPR011766	Thiamine pyrophosphate enzyme, TPP-binding	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.