Annotations: 8BC8

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	E set domains	8055093	3000070	SCOP2B (2022-06-29)
A [auth B]	SCOP2B Superfamily	Brl domain-like	8036193	3000115	SCOP2B (2022-06-29)
B [auth J]	SCOP2B Superfamily	JAB1/MPN domain-like	8053277	3001105	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	F_UNCLASSIFIED	e8bc8B1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: F_UNCLASSIFIED	ECOD (1.6)
A [auth B]	F_UNCLASSIFIED	e8bc8B7	A: alpha arrays	X: HTH	H: HTH	T: winged	F: F_UNCLASSIFIED	ECOD (1.6)
A [auth B]	F_UNCLASSIFIED	e8bc8B8	A: alpha arrays	X: HhH/H2TH	H: SAM-like subdomain in Sec63-like proteins (From Topology)	T: SAM-like subdomain in Sec63-like proteins	F: F_UNCLASSIFIED	ECOD (1.6)
A [auth B]	F_UNCLASSIFIED	e8bc8B11	A: alpha arrays	X: Sec63 N-terminal subdomain-like (From Topology)	H: Sec63 N-terminal subdomain-like (From Topology)	T: Sec63 N-terminal subdomain-like	F: F_UNCLASSIFIED	ECOD (1.6)
A [auth B]	F_UNCLASSIFIED	e8bc8B5	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: F_UNCLASSIFIED	ECOD (1.6)
B [auth J]	F_UNCLASSIFIED	e8bc8J1	A: a+b three layers	X: Cytidine deaminase-like (From Topology)	H: Cytidine deaminase-like (From Topology)	T: Cytidine deaminase-like	F: F_UNCLASSIFIED	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B]	PF02889	Sec63 Brl domain (Sec63)	Sec63 Brl domain	-	Family
A [auth B]	PF00270	DEAD/DEAH box helicase (DEAD)	DEAD/DEAH box helicase	Members of this family include the DEAD and DEAH box helicases. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome ...	Members of this family include the DEAD and DEAH box helicases. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.	Domain
A [auth B]	PF00271	Helicase conserved C-terminal domain (Helicase_C)	Helicase conserved C-terminal domain	The Prosite family is restricted to DEAD/H helicases, whereas this domain family is found in a wide variety of helicases and helicase related proteins. It may be that this is not an autonomously folding unit, but an integral part of the helicase.	Domain
B [auth J]	PF01398	JAB1/Mov34/MPN/PAD-1 ubiquitin protease (JAB)	JAB1/Mov34/MPN/PAD-1 ubiquitin protease	-	Family
B [auth J]	PF08084	PROCT (NUC072) domain (PROCT)	PROCT (NUC072) domain	The PROCT domain is the C-terminal domain in pre-mRNA splicing factors of PRO8 family [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	U5 small nuclear ribonucleoprotein 200 kDa helicase	catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity RNA binding binding organic cyclic compound binding nucleic acid binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity RNA binding binding organic cyclic compound binding nucleic acid binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity, acting on RNA RNA helicase activity ATP-dependent activity, acting on RNA identical protein binding protein binding	developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation ribonucleoprotein complex biogenesis RNA metabolic process mRNA processing protein-RNA complex assembly cellular component organization cellular component biogenesis nucleobase-containing compound metabolic process developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation ribonucleoprotein complex biogenesis RNA metabolic process mRNA processing protein-RNA complex assembly cellular component organization cellular component biogenesis nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process protein-RNA complex organization cellular component assembly protein-containing complex assembly cellular component organization or biogenesis RNA processing RNA splicing spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) protein-containing complex organization spliceosomal conformational changes to generate catalytic conformation primary metabolic process gene expression nucleobase-containing compound biosynthetic process mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process biosynthetic process RNA splicing, via transesterification reactions mRNA splicing, via spliceosome metabolic process RNA splicing, via transesterification reactions with bulged adenosine as nucleophile macromolecule metabolic process spliceosomal complex assembly mRNA cis splicing, via spliceosome cis assembly of pre-catalytic spliceosome	cellular anatomical structure membrane membrane-bounded organelle U2-type spliceosomal complex protein-containing complex intracellular anatomical structure catalytic complex nucleus nuclear protein-containing complex U2-type catalytic step 1 spliceosome intracellular membrane-bounded organelle intracellular organelle spliceosomal complex organelle cellular anatomical structure membrane membrane-bounded organelle U2-type spliceosomal complex protein-containing complex intracellular anatomical structure catalytic complex nucleus nuclear protein-containing complex U2-type catalytic step 1 spliceosome intracellular membrane-bounded organelle intracellular organelle spliceosomal complex organelle ribonucleoprotein complex catalytic step 1 spliceosome catalytic step 2 spliceosome spliceosomal snRNP complex Sm-like protein family complex U4/U6 x U5 tri-snRNP complex spliceosomal tri-snRNP complex small nuclear ribonucleoprotein complex membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen U2-type precatalytic spliceosome precatalytic spliceosome U5 snRNP
B [auth J]	Pre-mRNA-processing-splicing factor 8	RNA binding snRNA binding binding organic cyclic compound binding nucleic acid binding U1 snRNA binding U6 snRNA binding polyubiquitin modification-dependent protein binding protein binding modification-dependent protein binding K63-linked polyubiquitin modification-dependent protein binding U5 snRNA binding U2 snRNA binding pre-mRNA binding RNA binding snRNA binding binding organic cyclic compound binding nucleic acid binding U1 snRNA binding U6 snRNA binding polyubiquitin modification-dependent protein binding protein binding modification-dependent protein binding K63-linked polyubiquitin modification-dependent protein binding U5 snRNA binding U2 snRNA binding pre-mRNA binding pre-mRNA intronic binding	RNA splicing RNA metabolic process primary metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process nucleic acid biosynthetic process RNA splicing, via transesterification reactions biosynthetic process metabolic process cellular process RNA splicing RNA metabolic process primary metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process nucleic acid biosynthetic process RNA splicing, via transesterification reactions biosynthetic process metabolic process cellular process RNA processing macromolecule metabolic process ribonucleoprotein complex biogenesis mRNA processing spliceosomal tri-snRNP complex assembly protein-RNA complex assembly cellular component organization cellular component biogenesis protein-RNA complex organization cellular component assembly protein-containing complex assembly cellular component organization or biogenesis protein-containing complex organization spliceosomal snRNP assembly mRNA metabolic process mRNA splicing, via spliceosome RNA splicing, via transesterification reactions with bulged adenosine as nucleophile cellular response to stimulus biological process involved in interspecies interaction between organisms response to lipid response to stimulus response to other organism cellular response to lipopolysaccharide response to oxygen-containing compound response to external stimulus cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound response to biotic stimulus cellular response to biotic stimulus cellular response to chemical stimulus response to chemical response to bacterium cellular response to lipid response to external biotic stimulus response to molecule of bacterial origin response to peptide cellular response to tumor necrosis factor response to cytokine cellular response to cytokine stimulus response to tumor necrosis factor	cellular anatomical structure membrane membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear speck nuclear body intracellular organelle nucleoplasm organelle lumen organelle cellular anatomical structure membrane membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear speck nuclear body intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen U2-type spliceosomal complex protein-containing complex catalytic complex nuclear protein-containing complex U2-type catalytic step 1 spliceosome spliceosomal complex ribonucleoprotein complex catalytic step 1 spliceosome catalytic step 2 spliceosome spliceosomal snRNP complex Sm-like protein family complex U4/U6 x U5 tri-snRNP complex spliceosomal tri-snRNP complex small nuclear ribonucleoprotein complex U2-type precatalytic spliceosome precatalytic spliceosome U5 snRNP U2-type catalytic step 2 spliceosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B]	IPR048863	Pre-mRNA-splicing helicase BRR2-like, plug domain	Domain
A [auth B]	IPR041094	Brr2, N-terminal helicase PWI domain	Domain
A [auth B]	IPR004179	Sec63 domain	Domain
A [auth B]	IPR014756	Immunoglobulin E-set	Homologous Superfamily
A [auth B]	IPR011545	DEAD/DEAH box helicase domain	Domain
A [auth B]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A [auth B]	IPR050474	Helicase Hel308/SKI2-like	Family
A [auth B]	IPR001650	Helicase, C-terminal domain-like	Domain
A [auth B]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A [auth B]	IPR014001	Helicase superfamily 1/2, ATP-binding domain	Domain
A [auth B]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A [auth B]	IPR035892	C2 domain superfamily	Homologous Superfamily
B [auth J]	IPR012984	PROCT domain	Domain
B [auth J]	IPR012591	PRO8NT domain	Domain
B [auth J]	IPR019580	Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding	Domain
B [auth J]	IPR027652	Pre-mRNA-processing-splicing factor 8	Family
B [auth J]	IPR043172	Prp8 RNase domain IV, palm region	Homologous Superfamily
B [auth J]	IPR012592	PROCN domain	Domain
B [auth J]	IPR012337	Ribonuclease H-like superfamily	Homologous Superfamily
B [auth J]	IPR043173	Prp8 RNase domain IV, fingers region	Homologous Superfamily
B [auth J]	IPR021983	PRP8 domain IV core	Domain
B [auth J]	IPR019581	Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding	Domain
B [auth J]	IPR037518	MPN domain	Domain
B [auth J]	IPR019582	RNA recognition motif, spliceosomal PrP8	Domain
B [auth J]	IPR042516	Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily	Homologous Superfamily
B [auth J]	IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth B]	Pharos: O75643	retinitis pigmentosa retinitis pigmentosa 33 acute myeloid leukemia cone-rod dystrophy large cell medulloblastoma malignant pancreatic neoplasm osteosarcoma psoriasis astrocytic tumor dermatomyositis lung cancer medulloblastoma ovarian cancer retinal degeneration colorectal cancer retinitis pigmentosa retinitis pigmentosa 33 acute myeloid leukemia cone-rod dystrophy large cell medulloblastoma malignant pancreatic neoplasm osteosarcoma psoriasis astrocytic tumor dermatomyositis lung cancer medulloblastoma ovarian cancer retinal degeneration colorectal cancer diabetes mellitus malignant ependymoma oligodendroglioma primitive neuroectodermal tumor
B [auth J]	Pharos: Q6P2Q9	retinitis pigmentosa retinitis pigmentosa 13 HIV infectious disease Acute Cerebrovascular Accidents brain aneurysm cone-rod dystrophy malignant ependymoma pancreatic ductal adenocarcinoma acute myeloid leukemia diabetes mellitus oligodendroglioma retinal degeneration astrocytic tumor cerebrovascular disorder glioblastoma retinitis pigmentosa retinitis pigmentosa 13 HIV infectious disease Acute Cerebrovascular Accidents brain aneurysm cone-rod dystrophy malignant ependymoma pancreatic ductal adenocarcinoma acute myeloid leukemia diabetes mellitus oligodendroglioma retinal degeneration astrocytic tumor cerebrovascular disorder glioblastoma Hodgkins lymphoma ovarian cancer