SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 1mM RING-H2 finger domain of EL5 U-15N, 13C; 20mM Tris-HCl buffer, 100mM NaCl, 20uM ZnSO4, 1mM DTT; 90%H2O, 10% D2O | 90% H2O/10% D2O | 7.0 | ambient | 308 | ||
| 2 | 4D_13C/15N-separated_NOESY | 1mM RING-H2 finger domain of EL5 U-15N, 13C; 20mM Tris-HCl buffer, 100mM NaCl, 20uM ZnSO4, 1mM DTT; 90%H2O, 10% D2O | 90% H2O/10% D2O | 7.0 | ambient | 308 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry simulated annealing | XwinNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 15 |
| Representative Model | 15 (n/a) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XwinNMR | ||
| 2 | data analysis | NMRPipe | Deraglio | |
| 3 | refinement | X-PLOR | 3.1 | Brunger |
