RDC-derived models of the zinc ribbon domain of human general transcription factor TFIIB (zinc free structures)
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-15N HSQC | 0.6mM TFIIB Uniform labeling with 15N: U-98% 15N, 20mM [2H4]-imidazole hydrochloride, 5% glycerol and 20mM sodium sulfate at pH 6.5 | 95% H2O/5% D2O | 6.5 | ambient | 298 | ||
| 2 | 2D 1H-15N IPAP HSQC | 0.6mM TFIIB Uniform labeling with 15N: U-98% 15N, 20mM [2H4]-imidazole hydrochloride, 5% glycerol and 20mM sodium sulfate at pH 6.5, 12mg/mL of filamentous phage, Pf1 | 95% H2O/5% D2O | 6.5 | ambient | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | the structures are based on a total of 465 restraints, 443 are distance constraints, 22 1H-15N RDC restraints | VNMR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations, structures with the lowest energy |
| Conformers Calculated Total Number | 35 |
| Conformers Submitted Total Number | 35 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | VNMR | 6.1C | Varian |
| 2 | processing | NMRPipe | 2.1 | Frank Delaglio |
| 3 | data analysis | NMRView | 5.0 | Bruce A. Johnson |
| 4 | structure solution | CNS | 1.1 | Brunger |
| 5 | refinement | CNS | 1.1 | Brunger |
