Experiment: 2N58

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-1H NOESY	0.6 mM protein, 50 mM [U-100% 2H] DPC	90% H2O/10% D2O	50	5.7	ambient	298
2	2D 1H-13C HSQC	0.6 mM protein, 50 mM [U-100% 2H] DPC	90% H2O/10% D2O	50	5.7	ambient	298
3	2D 1H-1H TOCSY	0.6 mM protein, 50 mM [U-100% 2H] DPC	90% H2O/10% D2O	50	5.7	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	700

NMR Refinement
Method	Details	Software
na		CYANA

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (fewest violations)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CYANA	2.1	Guntert, Mumenthaler and Wuthrich
2	refinement	n.a.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.