KHG/KDPG aldolase

UniProtKB accession:  P0A955

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UniProtKB description:  Involved in the degradation of glucose via the Entner-Doudoroff pathway (PubMed:15659677, PubMed:1624451). Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate (PubMed:11342129, PubMed:17962400, PubMed:17981470). In vitro, can also catalyze the cleavage of 2-keto-4-hydroxy-4-(2'-pyridyl)butyrate (KHPB), 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), and of the substrate analogs 2-keto-3-deoxy-gluconate (KDG) and 2-keto-4-hydroxyoctonate (KHO) (PubMed:17962400). Can accept some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate and fluorobutanoate (PubMed:17981470). In addition to its KDPG aldolase activity, catalyzes the reversible cleavage of 2-keto-4-hydroxyglutarate (KHG) to glyoxylate and pyruvate (PubMed:1098660, PubMed:1339418, PubMed:4560498, PubMed:7016177). The enzyme is stereoselective for the S-enantiomer of KHG (PubMed:1098660, PubMed:4560498). Cleavage of KHG could serve in tricarboxylic acid (TCA) cycle regulation or, when operating in the reverse direction, in the detoxification of glyoxylate (Probable). Finally, also shows a high level of oxaloacetate beta-decarboxylase activity (PubMed:1339418, PubMed:4560498). The enzyme could have several physiological roles and function as a stress response protein in addition to its role in the catabolism of sugar acids (PubMed:15659677).