2CFP

Structural Evidence for Induced Fit and a Mechanism for Sugar/H(+) Symport in Lacy.

(2006) EMBO J 25: 2038

• PubMed: 16525509 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1038/sj.emboj.7601028
• Primary Citation of Related Structures:  
  2CFP, 2CFQ


• PubMed Abstract: 
  

  Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

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Organizational Affiliation: 

Department of Biological Sciences, Membrane Protein Crystallography Group, Imperial College London, London, UK.