4OTB | pdb_00004otb

4-OXALOCROTONATE TAUTOMERASE OBSERVED AS AN OCTODECAMER, RHOMBOHEDRAL CRYSTAL FORM

PDB DOI: https://doi.org/10.2210/pdb4OTB/pdb

Classification: ISOMERASE
Organism(s): Pseudomonas putida
Expression System: Escherichia coli
Mutation(s): No

Deposited: 1998-10-15 Released: 2001-08-01
Deposition Author(s): Taylor, A.B., Whitman, C.P., Hackert, M.L.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.50 Å
R-Value Free:
0.258 (Depositor)
R-Value Work:
0.224 (Depositor)
R-Value Observed:
0.224 (Depositor)

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Native and inhibitor complex structures of 4-oxalocrotonate tautomerase from Pseudomonas putida mt-2 (University of Texas at Austin-136 pages)

(1998) Thesis

Macromolecule Content

Total Structure Weight: 81.86 kDa
Atom Count: 5,422
Modelled Residue Count: 707
Deposited Residue Count: 744
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
4-OXALOCROTONATE TAUTOMERASE	A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	62	Pseudomonas putida	Mutation(s): 0 EC: 5.3.2 (PDB Primary Data), 5.3.2.6 (UniProt)
UniProt
Find proteins for Q01468 (Pseudomonas putida) Explore Q01468 Go to UniProtKB: Q01468
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q01468
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.50 Å
R-Value Free: 0.258 (Depositor)
R-Value Work: 0.224 (Depositor)
R-Value Observed: 0.224 (Depositor)

Space Group: H 3

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 87.4	α = 90
b = 87.4	β = 90
c = 254.6	γ = 120

Software Package:

Software Name	Purpose
AMoRE	phasing
X-PLOR	refinement
SDMS	data reduction
SCALEPACK	data scaling

Structure Validation

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Entry History

Deposition Data

Released Date: 2001-08-01

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2001-08-01
Type: Initial release
Version 1.1: 2008-04-26
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance
Version 1.3: 2023-12-27
Changes: Data collection, Database references
Version 1.4: 2024-04-03
Changes: Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.