7Z64 | pdb_00007z64

A GH18 from haloalkaliphilic bacterium unveils environment-dependent variations in the catalytic machinery of chitinases

PDB DOI: https://doi.org/10.2210/pdb7Z64/pdb

Classification: HYDROLASE
Organism(s): Chitinivibrio alkaliphilus ACht1
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2022-03-10 Released: 2024-01-17
Deposition Author(s): Madhuprakash, J., Dalhus, B., Rohr, A.K., Bissaro, B., Vaaje-Kolstad, G., Sorlie, M., Eijsink, V.G.
Funding Organization(s): Science and Engineering Research Board (SERB), Department of Science & Technology (DST, India)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.31 Å
R-Value Free:
0.166 (Depositor), 0.170 (DCC)
R-Value Work:
0.148 (Depositor), 0.150 (DCC)
R-Value Observed:
0.149 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

A GH18 from haloalkaliphilic bacterium unveils environment-dependent variations in the catalytic machinery of chitinases

Madhuprakash, J., Dalhus, B., Rohr, A.K., Bissaro, B., Vaaje-Kolstad, G., Sorlie, M., Eijsink, V.G.H.

To be published.

Macromolecule Content

Total Structure Weight: 64.88 kDa
Atom Count: 5,454
Modelled Residue Count: 556
Deposited Residue Count: 556
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Chitinase, GH18 family	A, B	278	Chitinivibrio alkaliphilus ACht1	Mutation(s): 0 Gene Names: CALK_0774 EC: 3.2.1.14
UniProt
Find proteins for U7D706 (Chitinivibrio alkaliphilus ACht1) Explore U7D706 Go to UniProtKB: U7D706
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	U7D706
Sequence Annotations Expand
Reference Sequence LOADING

Oligosaccharides

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Entity ID: 2
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	C, D	6		N/A	Interactions Focus chain C Focus chain D Interactions & Density Focus chain C Focus chain D
Glycosylation Resources
GlyTouCan: G36414FS GlyCosmos: G36414FS GlyGen: G36414FS

Small Molecules

Ligands 2 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
EDO Query on EDO Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain F [auth A] SDF format, chain G [auth A] SDF format, chain I [auth B] SDF format, chain J [auth B] SDF format, chain K [auth B] MOL2 format, chain E [auth A] MOL2 format, chain F [auth A] MOL2 format, chain G [auth A] MOL2 format, chain I [auth B] MOL2 format, chain J [auth B] MOL2 format, chain K [auth B] mmCIF format, chain E [auth A] mmCIF format, chain F [auth A] mmCIF format, chain G [auth A] mmCIF format, chain I [auth B] mmCIF format, chain J [auth B] mmCIF format, chain K [auth B]	E [auth A] F [auth A] G [auth A] I [auth B] J [auth B] E [auth A], F [auth A], G [auth A], I [auth B], J [auth B], K [auth B]	1,2-ETHANEDIOL C₂ H₆ O₂ LYCAIKOWRPUZTN-UHFFFAOYSA-N		Interactions Focus chain E [auth A] Focus chain F [auth A] Focus chain G [auth A] Focus chain I [auth B] Focus chain J [auth B] Focus chain K [auth B] Interactions & Density Focus chain E [auth A] Focus chain F [auth A] Focus chain G [auth A] Focus chain I [auth B] Focus chain J [auth B] Focus chain K [auth B]
NA Query on NA Download Ideal Coordinates CCD File SDF format, chain H [auth A] SDF format, chain L [auth B] MOL2 format, chain H [auth A] MOL2 format, chain L [auth B] mmCIF format, chain H [auth A] mmCIF format, chain L [auth B]	H [auth A], L [auth B]	SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N		Interactions Focus chain H [auth A] Focus chain L [auth B] Interactions & Density Focus chain H [auth A] Focus chain L [auth B]