8EWW | pdb_00008eww

Structure of Arabidopsis fatty acid amide hydrolase mutant S305A

PDB DOI: https://doi.org/10.2210/pdb8EWW/pdb

Classification: HYDROLASE
Organism(s): Arabidopsis thaliana
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2022-10-24 Released: 2024-05-29
Deposition Author(s): Aziz, M., Wang, X., Gaguancela, O.A., Chapman, K.D.
Funding Organization(s): National Science Foundation (NSF, United States)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free:
0.260 (Depositor), 0.260 (DCC)
R-Value Work:
0.234 (Depositor), 0.230 (DCC)
R-Value Observed:
0.235 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Structural interactions explain the versatility of FAAH in the hydrolysis of plant and microbial acyl amide signals

Aziz, M., Wang, X., Gaguancela, O.A., Chapman, K.D.

To be published.

Macromolecule Content

Total Structure Weight: 139.13 kDa
Atom Count: 9,228
Modelled Residue Count: 1,189
Deposited Residue Count: 1,272
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Fatty acid amide hydrolase	A, B	636	Arabidopsis thaliana	Mutation(s): 1 Gene Names: FAAH, At5g64440, T12B11.3 EC: 3.5.1.99
UniProt
Find proteins for Q7XJJ7 (Arabidopsis thaliana) Explore Q7XJJ7 Go to UniProtKB: Q7XJJ7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q7XJJ7
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.260 (Depositor), 0.260 (DCC)
R-Value Work: 0.234 (Depositor), 0.230 (DCC)
R-Value Observed: 0.235 (Depositor)

Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 72.451	α = 90
b = 81.324	β = 104.51
c = 133.18	γ = 90

Software Package:

Software Name	Purpose
Aimless	data scaling
PHENIX	refinement
PDB_EXTRACT	data extraction
XDS	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2024-05-29

Deposition Author(s):

Gaguancela, O.A.

Funding Organization	Location	Grant Number
National Science Foundation (NSF, United States)	United States	--

Revision History (Full details and data files)

Version 1.0: 2024-05-29
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.