8HEK | pdb_00008hek

Crystal Structure of Anti-CRISPR AcrIE2

PDB DOI: https://doi.org/10.2210/pdb8HEK/pdb

Classification: VIRAL PROTEIN
Organism(s): Pseudomonas phage JBD88a
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2022-11-08 Released: 2023-09-20
Deposition Author(s): Koo, J., Ka, D., Bae, E.
Funding Organization(s): Rural Development Administration, National Research Foundation (NRF, Korea)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.23 Å
R-Value Free:
0.199 (Depositor), 0.200 (DCC)
R-Value Work:
0.180 (Depositor), 0.180 (DCC)
R-Value Observed:
0.181 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Biochemical characterization of type I-E anti-CRISPR proteins, AcrIE2 and AcrIE4

Koo, J., Lee, G., Ka, D., Park, C., Suh, J.Y., Bae, E.

(2023) Appl Biol Chem 66: 51

DOI: https://doi.org/10.1186/s13765-023-00808-z

Macromolecule Content

Total Structure Weight: 10.43 kDa
Atom Count: 816
Modelled Residue Count: 87
Deposited Residue Count: 92
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
AcrIE2	A	92	Pseudomonas phage JBD88a	Mutation(s): 0
UniProt
Find proteins for L7P7Y7 (Pseudomonas phage JBD88a) Explore L7P7Y7 Go to UniProtKB: L7P7Y7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	L7P7Y7
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.23 Å
R-Value Free: 0.199 (Depositor), 0.200 (DCC)
R-Value Work: 0.180 (Depositor), 0.180 (DCC)
R-Value Observed: 0.181 (Depositor)

Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 26.96	α = 90
b = 47.351	β = 90
c = 56.37	γ = 90

Software Package:

Software Name	Purpose
HKL-2000	data scaling
HKL-2000	data collection
PHENIX	model building
PHENIX	refinement
PHASER	phasing
PDB_EXTRACT	data extraction

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2023-09-20

Deposition Author(s):

Funding Organization	Location	Grant Number
Rural Development Administration	United States	PJ01653401
National Research Foundation (NRF, Korea)	Korea, Republic Of	2020R1A4A1018890

Revision History (Full details and data files)

Version 1.0: 2023-09-20
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.