8X34 | pdb_00008x34

Human Kelch like-ECH Associated Protein 1 (Keap1)

PDB DOI: https://doi.org/10.2210/pdb8X34/pdb

Classification: GENE REGULATION
Organism(s): Homo sapiens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2023-11-11 Released: 2023-11-22
Deposition Author(s): Yilmaz, M., Gul, M., DeMirci, H.
Funding Organization(s): Other government

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.00 Å
R-Value Free:
0.329 (Depositor), 0.280 (DCC)
R-Value Work:
0.275 (Depositor), 0.280 (DCC)
R-Value Observed:
0.275 (Depositor)

Starting Model: experimental
View more details

wwPDB Validation 3D Report Full Report

This is version 2.1 of the entry. See complete history.

Literature

Human KEAP1 Kelch domain at ambient temperature

Yilmaz, M., Gul, M., DeMirci, H.

To be published.

Macromolecule Content

Total Structure Weight: 62.71 kDa
Atom Count: 4,441
Modelled Residue Count: 572
Deposited Residue Count: 572
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Kelch-like ECH-associated protein 1	A, B	286	Homo sapiens	Mutation(s): 0 Gene Names: KEAP1
UniProt & NIH Common Fund Data Resources
Find proteins for Q14145 (Homo sapiens) Explore Q14145 Go to UniProtKB: Q14145
PHAROS: Q14145 GTEx: ENSG00000079999
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q14145
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.00 Å
R-Value Free: 0.329 (Depositor), 0.280 (DCC)
R-Value Work: 0.275 (Depositor), 0.280 (DCC)
R-Value Observed: 0.275 (Depositor)

Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 75.644	α = 90
b = 75.825	β = 90
c = 218.656	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
Aimless	data scaling
CrysalisPro	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2023-11-22

Deposition Author(s):

Funding Organization	Location	Grant Number
Other government	Turkey	--

Revision History (Full details and data files)

Version 1.0: 2023-11-22
Type: Initial release
Version 2.0: 2024-07-17
Type: Coordinate replacement
Reason: Model completeness
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Other, Refinement description, Structure summary
Version 2.1: 2024-10-16
Changes: Structure summary

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.