5W7X

Crystal Structure of FHA domain of human APLF in complex with XRCC1 bisphospho peptide

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth D]	SCOP2B Superfamily	SMAD/FHA domain-like	8088232	3000137	SCOP2B (2022-06-29)
C [auth B]	SCOP2 Family	FHA domain	8088231	4002605	SCOP2 (2022-06-29)
C [auth B]	SCOP2 Superfamily	SMAD/FHA domain-like	8088232	3000137	SCOP2 (2022-06-29)
B [auth A]	SCOP2B Superfamily	SMAD/FHA domain-like	8088232	3000137	SCOP2B (2022-06-29)
D [auth C]	SCOP2B Superfamily	SMAD/FHA domain-like	8088232	3000137	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth D]	FHA_1	e5w7xD1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA_1	ECOD (1.6)
C [auth B]	FHA_1	e5w7xB1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA_1	ECOD (1.6)
B [auth A]	FHA_1	e5w7xA1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA_1	ECOD (1.6)
D [auth C]	FHA_1	e5w7xC1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth D]	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
C [auth B]	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
B [auth A]	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
D [auth C]	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth D], B [auth A], C [auth B], D [auth C]	PF17913	FHA domain (FHA_2)	FHA domain	This entry represents a divergent FHA domain which in PNK binds to phosphorylated segment of XRCC1 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth D], B [auth A], C [auth B], D [auth C]	Aprataxin and PNK-like factor	protein binding binding histone binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding DNA endonuclease activity endonuclease activity catalytic activity, acting on DNA nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on a nucleic acid protein binding binding histone binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding DNA endonuclease activity endonuclease activity catalytic activity, acting on DNA nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on a nucleic acid hydrolase activity catalytic activity DNA nuclease activity protein carrier chaperone histone chaperone activity molecular carrier activity 3'-5' exonuclease activity exonuclease activity ADP-D-ribose modification-dependent protein binding modification-dependent protein binding poly-ADP-D-ribose binding carbohydrate derivative binding DNA-(apurinic or apyrimidinic site) endonuclease activity protein folding chaperone cation binding ion binding metal ion binding	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process DNA repair-dependent chromatin remodeling cellular component organization chromatin organization chromatin remodeling cellular component organization or biogenesis protein localization localization protein localization to chromatin cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process DNA repair-dependent chromatin remodeling cellular component organization chromatin organization chromatin remodeling cellular component organization or biogenesis protein localization localization protein localization to chromatin cellular localization protein localization to chromosome protein localization to organelle cellular macromolecule localization macromolecule localization regulation of epithelial to mesenchymal transition developmental process regulation of biological process regulation of cellular process regulation of developmental process cellular developmental process biological regulation animal organ development regulation of cell differentiation mesenchyme development tissue development epithelial to mesenchymal transition cell differentiation anatomical structure development mesenchymal cell differentiation adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity regulation of immune effector process multicellular organismal process regulation of DNA recombination regulation of leukocyte activation regulation of cell activation B cell activation involved in immune response cell activation involved in immune response regulation of primary metabolic process nucleobase-containing compound metabolic process multicellular organism development leukocyte mediated immunity regulation of immune system process somatic diversification of immune receptors regulation of nucleobase-containing compound metabolic process somatic recombination of immunoglobulin genes involved in immune response system development immune response regulation of adaptive immune response DNA recombination primary metabolic process DNA metabolic process regulation of B cell mediated immunity immune effector process somatic diversification of immunoglobulins regulation of gene expression regulation of leukocyte mediated immunity biosynthetic process regulation of response to stimulus B cell mediated immunity regulation of immune response regulation of macromolecule biosynthetic process metabolic process regulation of production of molecular mediator of immune response macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process isotype switching immune system development immunoglobulin production immunoglobulin production involved in immunoglobulin-mediated immune response somatic cell DNA recombination lymphocyte activation leukocyte activation somatic diversification of immunoglobulins involved in immune response somatic recombination of immunoglobulin gene segments regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains regulation of immunoglobulin mediated immune response leukocyte activation involved in immune response regulation of B cell activation regulation of multicellular organismal process lymphocyte activation involved in immune response regulation of biosynthetic process production of molecular mediator of immune response regulation of isotype switching B cell activation cell activation regulation of macromolecule metabolic process gene expression adaptive immune response immunoglobulin mediated immune response macromolecule biosynthetic process nucleic acid metabolic process regulation of lymphocyte mediated immunity regulation of immunoglobulin production immune system process somatic diversification of immune receptors via germline recombination within a single locus regulation of lymphocyte activation multi-multicellular organism process reproductive process female pregnancy embryo implantation multi-organism reproductive process single strand break repair DNA repair positive regulation of biological process DNA ligation positive regulation of metabolic process regulation of DNA ligation positive regulation of DNA ligation positive regulation of macromolecule metabolic process positive regulation of cellular process positive regulation of nucleobase-containing compound metabolic process positive regulation of DNA metabolic process double-strand break repair double-strand break repair via nonhomologous end joining	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen site of double-strand break membraneless organelle site of DNA damage intracellular membraneless organelle chromosome
E [auth H], F [auth E], G [auth F], H [auth G]	DNA repair protein XRCC1	DNA binding oxidized DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding endonuclease activity catalytic activity, acting on a nucleic acid hydrolase activity 3' overhang single-stranded DNA endodeoxyribonuclease activity single-stranded DNA endodeoxyribonuclease activity DNA nuclease activity DNA endonuclease activity catalytic activity, acting on DNA DNA binding oxidized DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding endonuclease activity catalytic activity, acting on a nucleic acid hydrolase activity 3' overhang single-stranded DNA endodeoxyribonuclease activity single-stranded DNA endodeoxyribonuclease activity DNA nuclease activity DNA endonuclease activity catalytic activity, acting on DNA nuclease activity hydrolase activity, acting on ester bonds catalytic activity ADP-D-ribose modification-dependent protein binding protein binding modification-dependent protein binding poly-ADP-D-ribose binding carbohydrate derivative binding enzyme binding	response to stimulus response to chemical response to xenobiotic stimulus regulation of molecular function regulation of catalytic activity negative regulation of transferase activity biological regulation negative regulation of molecular function negative regulation of catalytic activity regulation of transferase activity negative regulation of protein ADP-ribosylation regulation of protein ADP-ribosylation cellular response to stimulus double-strand break repair response to stimulus response to chemical response to xenobiotic stimulus regulation of molecular function regulation of catalytic activity negative regulation of transferase activity biological regulation negative regulation of molecular function negative regulation of catalytic activity regulation of transferase activity negative regulation of protein ADP-ribosylation regulation of protein ADP-ribosylation cellular response to stimulus double-strand break repair DNA recombination primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process replication-born double-strand break repair via sister chromatid exchange cellular response to stress DNA damage response response to stress recombinational repair metabolic process cellular process macromolecule metabolic process double-strand break repair via homologous recombination DNA repair developmental process multicellular organismal process head development forebrain development animal organ development brain development nervous system development pallium development multicellular organism development central nervous system development anatomical structure development telencephalon development hippocampus development limbic system development system development voluntary musculoskeletal movement multicellular organismal movement musculoskeletal movement system process nervous system process neuromuscular process cerebellum morphogenesis hindbrain morphogenesis anatomical structure morphogenesis cerebellum development metencephalon development hindbrain development telomere maintenance in response to DNA damage negative regulation of telomere maintenance regulation of cellular process regulation of cellular response to stress negative regulation of biological process telomere organization regulation of primary metabolic process negative regulation of chromosome organization negative regulation of nucleobase-containing compound metabolic process negative regulation of telomere maintenance in response to DNA damage regulation of nucleobase-containing compound metabolic process regulation of biological process negative regulation of cellular component organization negative regulation of metabolic process regulation of chromosome organization regulation of organelle organization telomere maintenance telomere capping regulation of response to stimulus regulation of metabolic process regulation of DNA metabolic process regulation of telomere maintenance regulation of cellular component organization regulation of protection from non-homologous end joining at telomere cellular component organization regulation of response to stress negative regulation of cellular process cellular component organization or biogenesis negative regulation of macromolecule metabolic process regulation of telomere maintenance in response to DNA damage negative regulation of response to stimulus protection from non-homologous end joining at telomere chromosome organization regulation of macromolecule metabolic process negative regulation of protection from non-homologous end joining at telomere organelle organization negative regulation of organelle organization negative regulation of telomere capping negative regulation of DNA metabolic process regulation of telomere capping single strand break repair positive regulation of biological process positive regulation of single strand break repair positive regulation of response to stimulus positive regulation of metabolic process positive regulation of DNA repair positive regulation of macromolecule metabolic process positive regulation of cellular process positive regulation of nucleobase-containing compound metabolic process regulation of single strand break repair regulation of DNA repair positive regulation of DNA metabolic process mitotic recombination telomeric DNA-containing double minutes formation telomere maintenance via recombination base-excision repair response to decreased oxygen levels response to hypoxia response to oxygen levels response to abiotic stimulus response to oxidative stress response to oxygen-containing compound response to hydroperoxide DNA ligation positive regulation of molecular function regulation of ligase activity regulation of DNA ligation positive regulation of DNA ligation regulation of DNA ligase activity positive regulation of catalytic activity positive regulation of DNA ligase activity positive regulation of ligase activity double-strand break repair via nonhomologous end joining	membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure organelle nucleotide-excision repair complex ERCC4-ERCC1 complex membrane-enclosed lumen intracellular membraneless organelle nucleolus membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure organelle nucleotide-excision repair complex ERCC4-ERCC1 complex membrane-enclosed lumen intracellular membraneless organelle nucleolus intracellular organelle lumen organelle lumen membraneless organelle nuclear lumen nucleoplasm chromatin chromosome chromosome, telomeric region chromosomal region site of DNA damage

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth D], B [auth A], C [auth B], D [auth C]	IPR019406	Aprataxin and PNK-like factor, PBZ domain	Domain
A [auth D], B [auth A], C [auth B], D [auth C]	IPR041388	PNK, FHA domain	Domain
A [auth D], B [auth A], C [auth B], D [auth C]	IPR008984	SMAD/FHA domain superfamily	Homologous Superfamily
A [auth D], B [auth A], C [auth B], D [auth C]	IPR039253	Aprataxin and PNK-like factor	Family
E [auth H], F [auth E], G [auth F], H [auth G]	IPR008979	Galactose-binding-like domain superfamily	Homologous Superfamily
E [auth H], F [auth E], G [auth F], H [auth G]	IPR045080	XRCC1, first (central) BRCT domain	Domain
E [auth H], F [auth E], G [auth F], H [auth G]	IPR001357	BRCT domain	Domain
E [auth H], F [auth E], G [auth F], H [auth G]	IPR002706	DNA-repair protein Xrcc1, N-terminal	Domain
E [auth H], F [auth E], G [auth F], H [auth G]	IPR036420	BRCT domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth D], B [auth A], C [auth B], D [auth C]	Pharos: Q8IW19	celiac disease osteosarcoma lung carcinoma vascular disease nicotine dependence
E [auth H], F [auth E], G [auth F], H [auth G]	Pharos: P18887	esophageal squamous cell carcinoma mixed glioma prostate cancer spinocerebellar ataxia, autosomal recessive 26 Erythema stomatitis malignant glioma osteosarcoma cancer gastric neoplasm malignant mesothelioma mesothelioma neurodegenerative disease benign mesothelioma childhood acute lymphoblastic leukemia esophageal squamous cell carcinoma mixed glioma prostate cancer spinocerebellar ataxia, autosomal recessive 26 Erythema stomatitis malignant glioma osteosarcoma cancer gastric neoplasm malignant mesothelioma mesothelioma neurodegenerative disease benign mesothelioma childhood acute lymphoblastic leukemia glioma lung carcinoma schizophrenia xeroderma pigmentosum colorectal cancer gastric cancer hereditary diffuse gastric adenocarcinoma medulloblastoma

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
E [auth H], F [auth E], G [auth F], H [auth G]	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046
E [auth H], F [auth E], G [auth F], H [auth G]	TPO	Parent Component: THR RESID: AA0037 , AA0038 PSI-MOD : O-phospho-L-serine MOD:00046 , O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.