7AE9 | pdb_00007ae9

Crystal structure of mono-AMPylated HEPN(R46E) toxin in complex with MNT antitoxin

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DUF86	e7ae9A1	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: DUF86	ECOD (1.6)
B	DUF86	e7ae9B1	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: DUF86	ECOD (1.6)
C	DUF86	e7ae9C1	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: DUF86	ECOD (1.6)
D	DUF86	e7ae9D1	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: DUF86	ECOD (1.6)
E	NTP_transf_2	e7ae9E1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
F	NTP_transf_2	e7ae9F1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
G	NTP_transf_2	e7ae9G1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
H	NTP_transf_2	e7ae9H1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.120.580	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	bsu32300-like	CATH (utative)
B	1.20.120.580	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	bsu32300-like	CATH (utative)
C	1.20.120.580	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	bsu32300-like	CATH (utative)
D	1.20.120.580	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	bsu32300-like	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF01934	Ribonuclease HepT-like (HepT-like)	Ribonuclease HepT-like	-	Family
E, F, G, H	PF18765	Polymerase beta, Nucleotidyltransferase (Polbeta)	Polymerase beta, Nucleotidyltransferase	A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	HEPN toxin	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity binding nucleoside phosphate binding nucleotide binding heterocyclic compound binding small molecule binding catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity	-	protein-containing complex transcription regulator complex toxin-antitoxin complex transcription repressor complex
E, F, G, H	MNT ANTITOXIN	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding transferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups cation binding metal ion binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR052379	Type VII TA system RNase	Family
A, B, C, D	IPR008201	Ribonuclease HepT-like	Family
A, B, C, D	IPR037038	tRNA nuclease HepT-like superfamily	Homologous Superfamily
E, F, G, H	IPR041633	Polymerase beta, nucleotidyltransferase	Domain
E, F, G, H	IPR043519	Nucleotidyltransferase superfamily	Homologous Superfamily
E, F, G, H	IPR052930	Type VII TA system antitoxin MntA	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.