3J2M | pdb_00003j2m

The X-ray structure of the gp15 hexamer and the model of the gp18 protein fitted into the cryo-EM reconstruction of the extended T4 tail


ELECTRON MICROSCOPY
Sample
Bacteriophage T4 tail
Sample Components
Bacteriophage T4 tail terminator protein, gp15
Bacteriophage T4 tail sheath protein, gp18
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles3029
Reported Resolution (Å)15
Resolution MethodFSC 0.5 CUT-OFF
Other Detailsreconstruction done with in-house implementation of BP RP algorithm from SPIDER to support non-cubic reconstruction volumes
Refinement Type
Symmetry TypePOINT
Point SymmetryC6
Map-Model Fitting and Refinement
Id1
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsREFINEMENT PROTOCOL--rigid body
Data Acquisition
Imaging Experiment1
Date of Experiment2003-01-10
Temperature (Kelvin)
Microscope ModelFEI/PHILIPS CM300FEG/ST
Minimum Defocus (nm)1000
Maximum Defocus (nm)4000
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder ModelGATAN LIQUID NITROGEN
Nominal Magnification45000
Calibrated Magnification47000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGEMfit
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
each particle image